Citation: HE Mao-Fang, ZHANG Bo, TANG Yi-Mei, HAN Lu. Preparation of High Capacity Anion Exchange Magnetic Microspheres and Its Adsorption of Protein[J]. Chinese Journal of Analytical Chemistry, ;2018, 46(9): 1486-1492. doi: 10.11895/j.issn.0253-3820.181212 shu

Preparation of High Capacity Anion Exchange Magnetic Microspheres and Its Adsorption of Protein

College of Pharmacy, Institute of Drug Research, Xi'an Medical University, Xi'an 710021, China

Received Date: 31 March 2018
Revised Date: 19 July 2018

Fund Project: This work was supported by the Doctor Research Supporting Foundation (No. 2016D0C06) and the National Natural Science Foundation Supporting Program of Xi'an Medical University (No.2017GJFU08).

A high capacity anion exchange magnetic absorbent was prepared by immobilization of hyperbranched polyethyleneimine (PEI) on polydopamine-coated Fe₃O₄ microspheres (Fe₃O₄@pDA) and the ion exchange capacity was determined as 9.1 mmol/g. The absorbent was characterized by transmission electron microscope (TEM), infrared spectroscopy (IR) and thermogravimetric analysis (TGA). Moreover, the adsorption behavior of the absorbent for protein was carefully investigated by static adsorption using bovine serum albumin (BSA) and β-casein as models and then the static isotherms were determined under the optimum conditions. It was shown that the highest adsorption amount for protein was obtained when the adsorption time was 2 h and the pH was 7.0. Under the optimum conditions, the saturated adsorption capacity towards β-casein and BSA was determined as high as 237.5 and 204.5 μg/mg, respectively. Comparatively, the adsorbent exhibited little adsorption towards lysozyme and Rnase A, showing highly selectivity towards acidic protein. Therefore, the adsorbent exhibited potential in the separation and purification of protein from real samples.
- High capacity,
- Anion exchange,
- Polyethyleneimine,
- Protein,
- Separation and purification,
- Magnetic microspheres
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