Citation: WU Sheng-Nan, ZHANG Hao, JIANG Ling, HUO Feng-Wei, TIAN Dan-Bi. A Fluorescence Sensor for Determination of Lipase Activity Based on Curcumin-Hg²⁺ Complex[J]. Chinese Journal of Analytical Chemistry, ;2018, 46(10): 1588-1594. doi: 10.11895/j.issn.0253-3820.181187 shu

A Fluorescence Sensor for Determination of Lipase Activity Based on Curcumin-Hg²⁺ Complex

1.
College of Chemistry and Molecular Engineering, Nanjing Tech University, Nanjing 211816, China;
2.
College of Food Science and Light Industry, Nanjing Tech University, Nanjing 211816, China;
3.
Institute of Advanced Material, Nanjing Tech University, Nanjing 211816, China

Received Date: 22 March 2018
Revised Date: 14 August 2018

Fund Project: This work was supported by the Joint Funds of the National Natural Science Foundation of China (No. U1603112, 21574065) and the National Science Funds for Distinguished Young Scholars (No. 21625401).

A biosensor for detecting lipase activity was constructed by using the Tween 40-curcumin complex micelle as a fluorescence probe, Hg²⁺ as a quenching agent and methyl thioglycolate as the substrate of lipase. Hg²⁺ combined with the β-diketone structure of curcumin, resulting in quenching of Tween 40-curcumin complex micelle fluorescence. However, upon the addition of lipase, lipase could catalyze the hydrolysis of methyl thioglycolate to produce mercaptoacetic acid which could capture Hg²⁺ released from the curcumin-Hg²⁺ complex, resulting in the recovery of fluorescence of the Tween 40-curcumin complex micelle. The intensity of fluorescence recovery was related to lipase activity. The fluorescence sensing strategy was applied to quantitatively detect lipase activity. Under optimal experimental conditions, the change of fluorescence intensity was linear with the concentration of lipase in the range of 2-80 U/mL, and the detection limit was 0.01 U/mL. This method was employed to detect the activity of four other kinds of commercial lipases. The results were in agreement with those determined by potentiometric titration. The results showed that the method had the advantages of low cost, simple operation, high sensitivity, good practicability, and could be used for high throughput detection of lipase activity.
- Lipase activity,
- Tween 40-curcumin complex micelle,
- Mercury ion,
- Methyl thioglycolate,
- Fluorescence sensor
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通讯作者: 陈斌, bchen63@163.com

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沈阳化工大学材料科学与工程学院沈阳 110142

A Fluorescence Sensor for Determination of Lipase Activity Based on Curcumin-Hg2+ Complex

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通讯作者: 陈斌, bchen63@163.com

A Fluorescence Sensor for Determination of Lipase Activity Based on Curcumin-Hg²⁺ Complex