Citation: LIU Xiao-Ning, PEI Xing-Li, GONG Can, XU Xu. Matrix-assisted Laser Desorption Ionization-Mass Spectrometry Imaging of Small Molecules in Mulberry Leaf Using Ionic Liquid as Matrix[J]. Chinese Journal of Analytical Chemistry, ;2018, 46(12): 1923-1930. doi: 10.11895/j.issn.0253-3820.181144 shu

Matrix-assisted Laser Desorption Ionization-Mass Spectrometry Imaging of Small Molecules in Mulberry Leaf Using Ionic Liquid as Matrix

School of Chemical and Environmental Engineering, Shanghai Institute of Technology, Shanghai 201418, China

Received Date: 7 March 2018
Revised Date: 29 September 2018

Fund Project: This work was supported by the National Natural Science Foundation of China (No. 31671928) and the Natural Science Foundation of Shanghai (No. 15ZR1440800).

Ionic liquid matrix 2,5-dihydroxybenzoic acid/N,N-dimethylaniline (DHB/DMA) and ultrasonic spray deposition method were selected for matrix-assisted laser desorption ionization-mass spectrometry (MALDI-MS) analysis and MALDI-MS imaging of small molecule components on the surface of mulberry leaf and in leaf vein, including protocatechuic acid, chlorogenic acid, monosaccharide and disaccharide, astragalin, rutin, isoquercetin, cyanidin-3-O-glucoside, quercetin-3-O-6"-O-acetyl-β-D-glucopyranoside and kaempferol-3-O-rutinoside. The ultrasonic spray method was performed to deposit the ionic liquid matrix on sample slice with less diffusion of the analytes and improved reproducibility and sensitivity compared with spotting method and airbrush deposition method. The experimental conditions were optimized according to their effects on signal-to-noise ratio, and as a result, laser energy of 70%, ultrasonic spray 12 times, and 7.5 mmol/L additive KBr were selected. The distributions of different components on the surface of mulberry leaf or in leaf vein slice were analyzed by MALDI-MS imaging. The result suggested that the ionic liquid matrix and ultrasonic spray deposition matrix method could be well applied in MALDI-MS imaging of small molecular components in traditional Chinese medicine or plant.
- Mass spectrometry imaging,
- Ion liquid matrix,
- Mulberry leaf,
- Plant metabolite,
- Ultrasonic spray deposition
1. [1]
2. [2]
3. [3]
4. [4]
5. [5]
6. [6]
7. [7]
8. [8]
9. [9]
10. [10]
11. [11]
12. [12]
13. [13]
14. [14]
15. [15]
16. [16]
17. [17]
18. [18]
19. [19]
20. [20]
21. [21]
22. [22]
23. [23]
24. [24]
25. [25]
26. [26]
27. [27]
28. [28]
29. [29]
30. [30]
31. [31]
32. [32]
33. [33]
34. [34]
35. [35]
36. [36]
37. [37]
38. [38]
39. [39]
40. [40]
41. [41]
42. [42]
43. [43]
44. [44]
45. [45]
46. [46]
47. [47]
48. [48]
49. [49]
50. [50]
1. [1]
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