Citation: ZOU Xiao-Bo, SHI Yong-Qiang, ZHENG Yue, SHI Ji-Yong, HU Xue-Tao, JIANG Cai-Ping, HUANG Xiao-Wei, XU Yi-Wei. Detection of Arginine by AuNPs/CQDs Nanoprobes Based on Fluorescence Resonance Energy Transfer Effect[J]. Chinese Journal of Analytical Chemistry, ;2018, 46(6): 960-968. doi: 10.11895/j.issn.0253-3820.181096 shu

Detection of Arginine by AuNPs/CQDs Nanoprobes Based on Fluorescence Resonance Energy Transfer Effect

1.
School of Food and Biological Engineering, Jiangsu University, Zhenjiang 212013, China;
2.
Chinese Academy of Agricultural Mechanization Sciences, Beijing 100083, China

Received Date: 5 February 2018
Revised Date: 8 April 2018

Fund Project: This work was supported by the National Key Research and Development Program of China (No. 2016YFD0401104), the National Science and Technology Support Program (No. 2015BAD17B04), and the National Natural Science Foundation of China (No. 31671844).

A novel method for rapid detection of arginine based on fluorescence resonance energy transfer effect (FRET) between carbon quantum dots (CQDs) and gold nanoparticles (AuNPs) was developed. Firstly, the CQDs with excellent fluorescence properties were synthesized by one-step microwave assisted method. The AuNPs/CQDs composites were characterized and their quenching mechanism was analyzed. Then the amount of AuNPs/CQDs, the pH value and the reaction time were optimal. Under the optimum conditions, the fluorescence system was used to detect the content of arginine, showing a good linear relationship (R²=0.993) between fluorescence intensity and concentration of arginine in the range of 0.1-10.0 μmol/L, and the detection limit was 5.8 nmol/L. Finally, the content of arginine in grape juice was determined by this method with recoveries of 105.4%-110.8%, which indicated that the proposed FRET system had the potential for practical detection of arginine in fruit juices
- Arginine,
- fluorescent probe,
- Fluorescence resonance energy transfer,
- Carbon quantum dots,
- Gold nanoparticles
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沈阳化工大学材料科学与工程学院沈阳 110142