Citation: CHEN Yin-Juan, XIONG Xing-Chuang, LI Zhen-Hua, JIANG You, FANG Xiang, DAI Xin-Hua, DING Chuan-Fan. Investigation of Protonated and Lithiated Leucine-Enkephalin by Hydrogen/Deuterium Exchange and Theoretical Calculations[J]. Chinese Journal of Analytical Chemistry, ;2018, 46(4): 556-562. doi: 10.11895/j.issn.0253-3820.171479 shu

Investigation of Protonated and Lithiated Leucine-Enkephalin by Hydrogen/Deuterium Exchange and Theoretical Calculations

1.
School of Chemical Science and Engineering, Tongji University, Shanghai 200092, China;
2.
Department of Chemistry, Fudan University, Shanghai 200433, China;
3.
Chemical Metrology & Analytical Science Division, National Institute of Metrology, Beijing 100013, China

Received Date: 5 December 2017
Revised Date: 9 January 2018

Fund Project: This work was supported by the National Natural Science Foundation of China (Nos.21773035, 21605135) and the Ministry of Science and Techndogy of China (No.2012YQ22011307).

Differences of protonated and lithiated leucine-enkephalin(LE) were investigated by hydrogen deuterium exchange-mass spectrometry (HDX-MS) combined with quantum chemistry calculation. The results revealed that the protonated ions possessed very high product yield with all hydrogen atoms being exchanged, while the reaction of lithiated LE stopped after exchanging five hydrogen atoms in the same experimental conditions. The different HDX behaviours probably indicated their conformational differences. To further clarify the experimental results, the most stable conformations of protonated and lithiated leucine-enkephalin were calculated by density functional theory. It was found that terminal amino group was the most thermodynamically stable protonation site, while Li⁺ in coordination of four carbonyl oxygen atoms formed the most favourable lithiated LE. The reaction field reduction of lithium LE was probably due to the less acidity of hydrogen atoms and the increasing rigid conformation change induced by lithium ion.
- Hydrogen-deuterium exchange,
- Leucine-enkephalin,
- Mass spectrometry,
- Lithium ion,
- Gas-phase conformation
1. [1]
2. [2]
3. [3]
4. [4]
5. [5]
6. [6]
7. [7]
8. [8]
9. [9]
10. [10]
11. [11]
12. [12]
13. [13]
14. [14]
15. [15]
16. [16]
17. [17]
18. [18]
19. [19]
20. [20]
21. [21]
22. [22]
23. [23]
24. [24]
25. [25]
26. [26]
27. [27]
28. [28]
29. [29]
30. [30]
31. [31]
32. [32]
33. [33]
34. [34]
35. [35]
36. [36]
37. [37]
38. [38]
39. [39]
40. [40]
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沈阳化工大学材料科学与工程学院沈阳 110142