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Citation: LI Hui, LU Wen-Hui, JIAO Li-Li, WU Wei, SONG Feng-Rui, LIU Shu-Ying. Simultaneous Determination of Six Neurotransmitters in Rat Plasma, Adrenal Gland and Hypothalamus with Liquid Chromatography-Tandem Mass Spectrometry[J]. Chinese Journal of Analytical Chemistry, ;2018, 46(4): 586-593. doi: 10.11895/j.issn.0253-3820.171472 shu

Simultaneous Determination of Six Neurotransmitters in Rat Plasma, Adrenal Gland and Hypothalamus with Liquid Chromatography-Tandem Mass Spectrometry

LI Hui ^1,2, ,
LU Wen-Hui ² ,
JIAO Li-Li ² ,
WU Wei ² ,
SONG Feng-Rui ¹ ,
LIU Shu-Ying ^1,2

1.
Changchun Institute of Applied Chemistry Chinese Academy of Sciences, Changchun 130022, China;
2.
Changchun University of Chinese Medicine, Jilin Ginseng Academy, Changchun 130117, China

Received Date: 2 December 2017
Revised Date: 5 January 2018

A rapid Liquid chromatography-tandem mass spectrometry method was developed for the simultaneous determination of six neurotransmitters in rat plasma, adrenal gland and hypothalamus, including γ-aminobutyric acid (GABA), serotonin(5-HT), tyrosine(L-Tyr), dopamine(DA), norepinephrine(NE) and epinephrine(E). This method was used for the neurotransmitters detection in rat adrenal, blood and hypothalamus. The samples were pre-column derivatized with dansyl chloride, and 5-HTP and CA were used as internal standards. A Thermo C₁₈ column (50 mm×3 mm, 2.7 μm) was used for sample separation with 0.1% aqueous formic acid solution as phase A and methanol as phase B at a flow rate of 0.2 mL/min, and the injection volume was set at 2 μL. The detection was carried out with multi-reaction monitoring (MRM) in electron spray ionization (ESI) positive mode. The linear ranges for detection of GABA, 5-HT, L-Tyr, DA, NE and E were 0.26-620.80 μmol/L, 0.03-11.20 μmol/L, 1.20-88.00 μmol/L, 0.03-41.02 μmol/L, 0.01-47.20 μmol/L, 0.01-90.24 μmol/L, respectively, with recoveries varying from 91.16% to 116.20%. This method can detect the neurotransmitters rapidly and accurately, providing a platform for the determination of neurotransmitters in rat plasma, adrenal gland and hypothalamus in pharmacological experiments.
- Liquid chromatography-tandem mass spectrometry,
- Adrenal,
- Blood,
- Hypothalamus,
- Neurotransmitters
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