Citation: YU Jia-Jun, LIU Ping, ZENG Zhen, CHEN Ying, GAO Wei, LI Mei, WANG Chen-Guang, HUANG Zheng-Xu, ZHOU Zhen, LI Lei. Development and Characterization of A Linear Matrix-assisted Laser Desorption Ionization Mass Spectrometer[J]. Chinese Journal of Analytical Chemistry, ;2018, 46(4): 463-470. doi: 10.11895/j.issn.0253-3820.171415 shu

Development and Characterization of A Linear Matrix-assisted Laser Desorption Ionization Mass Spectrometer

YU Jia-Jun ^1, ,
LIU Ping ³ ,
ZENG Zhen ¹ ,
CHEN Ying ¹ ,
GAO Wei ^1,2 ,
LI Mei ^1,2 ,
WANG Chen-Guang ⁴ ,
HUANG Zheng-Xu ^1,2 ,
ZHOU Zhen ^1,2 ,
LI Lei ^1,2

1.
Institute of Mass Spectrometer and Atmospheric Environment, Jinan University, Guangzhou 510632, China;
2.
Guangdong Provincial Engineering Research Center for On-line Source Apportionment System of Air Pollution, Guangzhou 510632, China;
3.
Guangzhou HeXin Instrument Co., LTD, Guangzhou 510530, China;
4.
Guangzhou Institute of Energy Conversion, Chinese Academy of Sciences, Guangzhou 510640, China

Received Date: 11 November 2017
Revised Date: 1 December 2017

Matrix-assisted laser desorption ionization time-of-flight mass spectrometry (MALDI-TOF MS) is an important analytical technique for biological macromolecules, such as proteins, peptides and nucleic acid, especially in the field of microbial identification. Based on previous study, a linear MALDI-TOF MS has been designed and assembled for biological applications. The instrument comprises a vacuum system, a vacuum fast sample introduction system, an optical system, a time-of-flight mass analyzer, an ion source, a data acquisition system and an electric control system. The ion source adopts two-stage source acceleration, delayed extraction and dynamic pulse focusing technology. The time-of-flight distance of field-free drift region is about 1 meter. The optical system adopts a solid laser with adjustable frequency of 1-2000 Hz and spots of 20-100 μm. The angle of incidence laser is controlled at 5 degrees. A series of experiments are carried out to further evaluate the instrument performances. It can not only analyze the samples more than 199 kDa, but also achieve isotope resolution at 1000-3000 Da and up to 900(FWHM) at 5000-17000 Da. The minimum detectable concentration of gramicidin is 10 amol/μL, absolute sensitivity can reach up to 2.56 amol. Independent detection of saliva samples from different targets shows that the instrument has high reproducibility. We identified Escherichia Coli and Shigella spp, which are two common bacteria but difficult to be differentiated by mass spectrometry, showing its potential identification for clinical microorganism. In a summary, this instrument can play a role on clinical examination in the near future.
- Matrix-assisted laser desorption ionization,
- Linear time-of-flight mass spectrometer,
- Development,
- Performance characterization
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