Citation: XIA Kai-Min, WENG Guo-Feng, WANG Zhi-Peng, WANG Fang-Jun, XIAO Chun-Lei, PAN Yuan-Jiang, WU Jun. Analysis of Steroid Hormone by Low Temperature Plasma Mass Spectrometry[J]. Chinese Journal of Analytical Chemistry, ;2017, 45(12): 1944-1950. doi: 10.11895/j.issn.0253-3820.171401 shu

Analysis of Steroid Hormone by Low Temperature Plasma Mass Spectrometry

1.
Department of Chemistry, Zhejiang University, Hangzhou 310027, China
2.
CAS Key Laboratory of Separation Sciences for Analytical Chemistry, Dalian Institute of Chemical Physics, Chinese Academy of Sciences, Dalian 116023, China

Corresponding author: PAN Yuan-Jiang, panyuanjiang@zju.edu.cn WU Jun, wujunwu@zju.edu.cn
Received Date: 6 November 2017
Accepted Date: 15 November 2017

Fund Project: the National Natural Science Foundation of China 21372199This work was supported by the National Natural Science Foundation of China (No. 21372199)

Figures(5)

A device to produce low temperature plasma (LTP) was designed and constructed to serve as the ion source of a high resolution mass spectrometry, and was applied to qualitatively analyze the steroid samples. In comparison with conventional electrospray ionization mass spectrometry, low temperature plasma mass spectrometry (LTP-MS) had some advantages such as simple sample pretreatment and less interference. Mass spectrometry and tandem mass spectrometry were used to characterize the steroid samples in this research, and it was found that the structural stability of each steroid sample was presented in its mass spectrum, while in the tandem mass spectra there were more fragments of H₂O lost. And then the fragmentation process of typical steroid samples in collision induced dissociation (CID) was discussed based on theoretical calculation. In addition, by comparing tandem mass spectrometry and the fragmentation process, a pair of isomers of testosterone and dehydroepiandrosterone could be distinguished successfully.
- Steroid,
- Low temperature plasma,
- Mass spectrometry,
- Isomer
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沈阳化工大学材料科学与工程学院沈阳 110142