Citation: WANG Zhong-Hua, HE Bing-Shu, SUN Cheng-Long, SONG Xiao-Wei, HE Jiu-Ming, ZHANG Rui-Ping, ABLIZ Zeper. Study on Tissue Distribution of A Variety of Endogenous Metabolites by Air Flow Assisted Ionization-Ultra High Resolution Mass Spectrometry Imaging[J]. Chinese Journal of Analytical Chemistry, ;2018, 46(3): 406-412. doi: 10.11895/j.issn.0253-3820.171348 shu

Study on Tissue Distribution of A Variety of Endogenous Metabolites by Air Flow Assisted Ionization-Ultra High Resolution Mass Spectrometry Imaging

1.
Centre for Imaging & Systems Biology, College of Life and Environmental Sciences, Minzu University of China, Beijing 100081, China;
2.
State Key Laboratory of Bioactive Substances and Functions of Natural Medicines, Institute of Materia Medica, Chinese Academy of Medical Sciences and Peking Union Medical College, Beijing 100050, China

Received Date: 17 October 2017
Revised Date: 2 January 2018

Fund Project: This work was supported by the Leading Talent Support Program of State Ethnic Affairs Commission, Excellent Youth Talent Support Project of Minzu University of China, and Program of Collaborative Innovation Centre for Ethnic Minority Development of Minzu University of China (No. 2017MDYQ13).

As a promising new molecular imaging technique, mass spectrometry imaging (MSI) has attracted more and more attention in the field of biomedicine. A method of air flow assisted ionization-ultra high resolution mass spectrometry-based mass spectrometric imaging (AFAI-MSI) was developed to profile endogenous metabolites in rat kidney tissue in this study. Rat kidneys were collected and cut into frozen tissue sections, and then were analyzed on an AFAI-MSI system in positive ion mode using acetonitrile-isopmpyl alcohol-water (4:4:2, V/V, 5 μL/min) as spray solvent, N₂ as spray gas (0.6 MPa) and air as assisting gas (45 L/min). The mass range and resolution were set to be 70-1000 Da and 70000, respectively. As a result, a total of 38 metabolites, including organic amines, sugars, vitamins, peptides, neurotransmitters, organic acids, phospholipids, sphingolipids, glyceride, and cholesterol esters, were identified and imaged to characterize their tissue-specific distribution in kidney tissues, and some metabolites, such as choline, acetylcoline, betaine, phoshocholine, and glycerophosphocholine were found to have distinct distribution along the cortex-medulla axis, which may be involved in the formation of osmotic pressure gradient in the kidney. The proposed ultra high resolution mass spectrometry based AFAI-MSI method could work without sample pretreatment, showed high sensitivity and wide metabolite coverage, and was expected to provide a new analytical approach for the research of in situ characterization and metabolic regulation mechanism of endogenous metabolites in kidney.
- Air flow assisted ionization,
- Mass spectrometric imaging,
- Endogenous metabolites,
- Kidney
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