Citation: CAO Ting-Ting, SUN Jun-Yong, GAO Feng. A Ratiometric Fluorescence Sensing for Carboxylesterase Based on Semiconducting Polymer Quantum Dots[J]. Chinese Journal of Analytical Chemistry, ;2017, 45(12): 1930-1936. doi: 10.11895/j.issn.0253-3820.171320 shu

A Ratiometric Fluorescence Sensing for Carboxylesterase Based on Semiconducting Polymer Quantum Dots

College of Chemistry and Materials Science, Anhui Normal University, Wuhu 241000, China

Corresponding author: GAO Feng, fgao@mail.ahnu.edu.cn
Received Date: 10 October 2017
Accepted Date: 4 November 2017

Fund Project: This work was supported by the National Natural Science Foundation of China (No. 21575004)the National Natural Science Foundation of China 21575004

Figures(6)

A carboxylesterase (CaE) ratiometric fluorescent probe based on semiconducting Poly(9, 9-dioctylfluorenyl-2, 7-diyl) (PFO) polymer quantum dots (Pdots) was prepared. The negatively charged PFO Pdots and the positively charged polyethyleneimine (PEI) formed a nanocomposite Pdots@PEI by electrostatic interaction, and emitted fluorescence at 440 nm and 467 nm. In the presence of CaE, the substrate fluorescein diacetate (FDA) was hydrolyed into negatively charged fluorescein molecule, and could get closer to the positively charged Pdots@PEI due to electrostatic interactions, which could induce resonance energy transfer, resulting in the energy donor Pdots@PEI fluorescence intensity gradually weakened and the fluorescence intensity of the FDA hydrolysis product increased. Based on the fluorescence intensity ratio of Pdots@PEI and the fluorescence enhancement of FDA hydrolysis products, a new method for selective detection of CaE was established. Under the optimal conditions, the linear range of the method on CaE testing was 0.75-50 U/L with detection limits of 0.75 U/L (S/N=3). This method was used for the detection of the content of CaE in rabbit blood with satisfactory results.
- Semiconducting polymer dots,
- Ratiometric fluorescent sensor,
- Carboxylesterase
1. [1]
  Park S J, Lee H W, Kim H R, Kang C H, Kim H M. Chem. Sci., 2016, 7:3703-3709 doi: 10.1039/C5SC05001D
2. [2]
  Li B, Sedlacek M, Manoharan I, Boopathy R, Duysen E G, Masson P, Lockridge O. Biochem. Phar., 2005, 70:1673-1684 doi: 10.1016/j.bcp.2005.09.002
3. [3]
  Yang Y, Huang Y Y, Zhang G X, Zhao R, Zhang D Q. Acta Chim. Sinica, 2016, 74:871-876 doi: 10.6023/A16080415
4. [4]
  Levine M N, Hoang T T, Raines R T. Chem.Biol., 2013, 20:614-618 doi: 10.1016/j.chembiol.2013.03.016
5. [5]
  Zhang Y Y, Chen W, Feng D, Shi W, Li X H, Ma H M. Analyst, 2012, 137:716-721 doi: 10.1039/C2AN15952J
6. [6]
  Koitka M, Hochel J, Obst D, Rottmann A, Gieschen H, Borchert H H. Anal. Biochem., 2008, 381:113-122 doi: 10.1016/j.ab.2008.06.019
7. [7]
  Sun J Y, Mei H, Wang S F, Gao F. Anal. Chem., 2016, 88:7372-7377 doi: 10.1021/acs.analchem.6b01929
8. [8]
  Cui Q L, Yang Y, Yao C, Liu R H, Li L D. ACS Appl. Mater. Interfaces, 2016, 8:35578-35586 doi: 10.1021/acsami.6b12525
9. [9]
  Xue Y Y, Liang W S, Li Y, Wu Y, Peng X W, Qiu X Q, Liu J B, Sun R C. J. Agr. Food Chem., 2016, 64:9592-9600 doi: 10.1021/acs.jafc.6b04583
10. [10]
  Chan Y H, Wu C F, Ye F M, Jin Y H, Smith P B, Chiu D T. Anal. Chem., 2011, 83:1448-1455 doi: 10.1021/ac103140x
11. [11]
  Zhang X J, Yu J B, Wu C F, Jin T H, Rong Y, Ye F M, Chiu D T. ACS Nano, 2012, 6(6):5429-5439 doi: 10.1021/nn301308w
12. [12]
  Wang C, Sun J Y, Mei H, Gao F. Anal. Methods, 2016, 8:7755-7761 doi: 10.1039/C6AY02082H
13. [13]
  Sun J Y, Wang S F, Gao F. Langmuir, 2016, 32:12725-12731 doi: 10.1021/acs.langmuir.6b03002
14. [14]
  Sun W, Hayden S, Jin Y H, Rong Y, Yu J B, Ye F M, Chan Y H, Zeigler M, Wu C F, Chiu D T. Nanoscale, 2012, 4:7246-7249 doi: 10.1039/c2nr32055j
15. [15]
  Wu C F, Bull B, Szymanski C, Christensen K, McNeill J. ACS Nano, 2008, 2(11):2415-2423 doi: 10.1021/nn800590n
16. [16]
  Wu C F, Zheng Y L, Szymanski C, McNeill J. J. Phys. Chem. C, 2008, 112:1772-1781 doi: 10.1021/jp074149+
17. [17]
  Clafton S N, Beattie D A, Agnieszka M V, Acres R G, Morgan A C, Kee T W. Langmuir, 2010, 26(23):17785-17789 doi: 10.1021/la103063p
18. [18]
  Gui R J, An X Q, Huang W X. Anal. Chim. Acta, 2013, 767:134-140 doi: 10.1016/j.aca.2013.01.006
19. [19]
  Yue X, Peng L, Li P C.H. Anal. Chem., 2004, 76:5282-5292 doi: 10.1021/ac0493830
20. [20]
  Gudiukaite R, Sadauskas M, Gegeckas A, Malunavicius V, Citavicius D. J. Ind. Microbiol. Biotechnol., 2017, 44:799-815 doi: 10.1007/s10295-017-1905-4
21. [21]
  Wang C H, Qiu J G, Yang Y J, Zheng J W, He J, Li S P. Biotechnol. Lett., 2017, 39:553-560 doi: 10.1007/s10529-016-2276-z
22. [22]
  Sigolaeva L V, Dubacheva G V, Porus M V, Eremenko A V, Rudakova E V, Makhaeva G F, Richardsond R J, Kurochkin I N. Anal. Methods, 2013, 5:3872-3879 doi: 10.1039/c3ay40339d
23. [23]
  Komatsu T, Urano Y, Fujikawa Y, Kobayashi T, Kojima H, Terai T, Hanaoka K, Nagano T. Chem. Commun., 2009:7015-7017
24. [24]
  Gao M, Hu Q L, Feng G X, Tang B Z, Liu B. J. Mater. Chem. B, 2014, 2:3438-3442 doi: 10.1039/C4TB00345D
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沈阳化工大学材料科学与工程学院沈阳 110142

A Ratiometric Fluorescence Sensing for Carboxylesterase Based on Semiconducting Polymer Quantum Dots

南开大学师唯/华北电力大学（保定）刘景维：二维配位聚合物中有序的亲锂冠醚位点用于无枝晶锂沉积

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通讯作者: 陈斌, bchen63@163.com