Citation: WANG Hong-Ya, YIN Bin-Cheng, YE Bang-Ce. Specific microRNA Detection Based on Surface Plasmon-Enhanced Energy Transfer Between Gold Nanoparticles and Silver Nanoclusters[J]. Chinese Journal of Analytical Chemistry, ;2017, 45(12): 2018-2025. doi: 10.11895/j.issn.0253-3820.171317 shu

Specific microRNA Detection Based on Surface Plasmon-Enhanced Energy Transfer Between Gold Nanoparticles and Silver Nanoclusters

1.
School of Chemistry and Chemical Engineering, Shihezi University, Shihezi 832000, China
2.
Lab of Biosystem and Microanalysis, State Key Laboratory of Bioreactor Engineering, East China University of Science & Technology, Shanghai 200237, China

Corresponding author: YE Bang-Ce, bcye@ecust.edu.cn
Received Date: 11 October 2017
Accepted Date: 1 November 2017

Fund Project: This work was supported by the National Natural Science Foundation of China (Nos. 21675052, 21335003, 21575089) and the Fundamental Research Funds for the Central Universities, Chinathe National Natural Science Foundation of China 21335003the National Natural Science Foundation of China 21675052the National Natural Science Foundation of China 21575089

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There is high demand for a sensitive method for miRNA detection in clinical diagnosis. In this work, we developed a method for miRNA detection based on the surface plasmon-enhanced energy transfer (SPEET) between gold nanoparticles (AuNPs) and silver nanoclusters (AgNCs), coupled with DNA polymerase and nicking enzyme-assisted isothermal amplification for target recycling. Two DNA probes (Probe a and Probe b) were assembled onto the surface of AuNPs to form Probe b-Probe a-AuNP conjugates. Probe a consisted three domains: the complementary sequence of miRNA, the specific site of the nicking enzyme, and the self-assembly sequence for AgNCs. The 3' end of Probe a was modified with thiol as a binding site for AuNPs. The SPEET of AgNCs and AuNPs was inhibited when miRNA was added to produce the dumbbell shaped template by polymerase. The template could promote synthesis of AgNCs, resulting in replacement and subsequently recycling of the target molecule for signal amplification. In comparison with the traditional method of miRNA detection with commercial RT-PCR kits, this method avoided the process of reverse transcription and was easy to perform. In addition, this method with a detection limit of 2.5×10^-11 mol/L was cost-effective, label-free, and highly selective for detecting miRNA, and could be applied to the analysis of miRNA in biological samples.
- MicroRNA detection,
- Gold nanoparticles,
- Silver nanoclusters,
- Surface plasmon-enhanced energy transfer
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沈阳化工大学材料科学与工程学院沈阳 110142