Citation: MA Xu-Yan, QIN Yin-Hui, QI Chu-Bo, WU Ling-Ling, ZHANG Zhi-Ling, XIA He-Shun, PANG Dai-Wen. Immunomagnetic Nanosphere-based Strategy for Rapid, Efficient and Accurate Detection of Lymphnode Metastatic Carcinoma Cells[J]. Chinese Journal of Analytical Chemistry, ;2017, 45(12): 1874-1881. doi: 10.11895/j.issn.0253-3820.171298 shu

Immunomagnetic Nanosphere-based Strategy for Rapid, Efficient and Accurate Detection of Lymphnode Metastatic Carcinoma Cells

1.
Key Laboratory of Analytical Chemistry for Biology and Medicine(Ministry of Education), College of Chemistry and Molecular Sciences, State Key Laboratory of Virology, The Institute for Advanced Studies, Wuhan Institute of Biotechnology, Wuhan University, Wuhan 430072, China
2.
Hubei Cancer Hospital, Wuhan 430079, China

Corresponding author: XIA He-Shun, xiaheshun@sina.com PANG Dai-Wen, dwpang@whu.edu.cn
Received Date: 7 October 2017
Accepted Date: 7 November 2017

Fund Project: This work was supported by the National Natural Science Foundation of China (No.21535005)the National Natural Science Foundation of China 21535005

Figures(5)

A strategy based on immunomagnetic nanospheres (IMNs) for rapid, efficient and accurate detection of lymphnode metastasis carcinoma cells (LNMCCs) was developed in this study. First, IMNs processing magnetism and biological targeting were fabricated by the approach developed by our group previously. Then, LNMCCs in lymph node fine needle aspiration (LNFNA) specimens were separated and enriched by the immunomagnetic isolation using IMNs. At last, the captured cells were identified with Wright's staining and immunocytochemistry (ICC). The separation and enrichment of LNMCCs with immunomagnetic isolation could reduce the background interference of LNFNA specimens effectively; the identification with Wright's staining and ICC offered more reliable information for accurate diagnosis, so the sensitivity, specificity and overall diagnostic accuracy had an obvious improvement compared with the conventional cytologic diagnosis. Besides, the simple and rapid incubation of LNFNA specimens with IMNs needed just 5 min, so the cytomorphology of captured LNMCCs could be intactly retained, which enabled to provide important basis for classifying lymphnode metastasis carcinoma (LNMC) and the subsequent pathological study. Moreover, the specific capture of epithelial carcinoma cells in LNFNA specimens with IMNs could make a definite diagnosis of the captured cells as LNMCCs, thus realizing the differentiated diagnosis of LNMC and malignant lymphoma. Additionally, this strategy exhibited successful LNMCCs detection in LNFNA specimens from 110 patients and had higher sensitivity (98.0%), specificity (100.0%), and overall diagnostic accuracy (98.2%) than the conventional cytologic diagnosis. Therefore, it was a new attempt to use IMNs for detection of LNMCCs in LNFNA specimens from LNMC patients, and offered new ideas for LNMC diagnosis and study.
- Immunomagnetic nanospheres,
- Detection,
- Lymphnode metastasis carcinoma cells
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