Citation: ZHENG Si-Jia, WANG Qing-Qing, WANG Xiao-Lin, ZHAO Xin-Jie, XU Guo-Wang. Hydrophilic Interaction Liquid Chromatography Coupled with Mass Spectrometry for Serum Metabolomics Analysis of Bladder Cancer[J]. Chinese Journal of Analytical Chemistry, ;2017, 45(12): 1921-1929. doi: 10.11895/j.issn.0253-3820.171286 shu

Hydrophilic Interaction Liquid Chromatography Coupled with Mass Spectrometry for Serum Metabolomics Analysis of Bladder Cancer

1.
Chinese Academy Sciences Key Laboratory of Separation Science for Analytical Chemistry, Dalian Institute of Chemical Physics, Chinese Academy of Sciences, Dalian, 116023, China
2.
University of Chinese Academy of Sciences, Beijing 100049, China

Corresponding author: ZHAO Xin-Jie, xj_zhao1@126.com
Received Date: 2 October 2017
Accepted Date: 1 November 2017

Fund Project: the National Natural Science Foundation of China 21575140This work was supported by the National Natural Science Foundation of China (No. 21575140)

Figures(5)

Bladder cancer (BC) is a fatal malignancy with considerable mortality, and can cause a serious threat to human health. The successful treatment of bladder cancer relies mainly on early detection. Biomarkers are vital to early diagnosis of bladder cancer, and metabonomics play an important role in biomarkers finding. In this study, we used 69 polar metabolites to select the appropriate separation system and develop the zwitterionic hydrophilic chromatography/mass spectrometry (ZIC-HILIC/MS) method. In this method, 50 representative compounds had broad linear ranges between 2-6 orders of magnitude. Moreover the limit of detection of the method was below ng/mL levels. The analysis for six serum samples prepared in parallel showed that this method had good reproducibility, and the RSDs of more than 85% metabolites were less than 30%. Based on this method, it was found that 35 metabolites had significant differences in BC group and healthy control. After screening and validation, the combination of chenodeoxycholic acid, eicosenoic acid, GPC, dodecenoic acid and cystine was a potential biomarker to distinguish BC and normal group. These results indicated that the ZIC-HILIC/MS method could detect diverse metabolites for metabolomic analysis purpose with good reproducibility and stability.
- Zwitterionic hydrophilic interaction liquid chromatography/mass spectrometry,
- Metabolomics,
- Bladder cancer,
- Polar metabolites
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沈阳化工大学材料科学与工程学院沈阳 110142