Citation: FANG Chen, ZHENG Li-Juan, ZHAI Su-Yan, TU Yi-Feng. An Electrochemiluminescent Method to Detect A Dipeptide Related to Diabetes[J]. Chinese Journal of Analytical Chemistry, ;2017, 45(12): 1951-1955. doi: 10.11895/j.issn.0253-3820.171284 shu

An Electrochemiluminescent Method to Detect A Dipeptide Related to Diabetes

1.
Institute of Analytical Chemistry, Dushu Lake Campus, Soochow University, Industrial Park, Suzhou 215123, China
2.
Department of Endocrinology, The Second Affiliated Hospital of Soochow University, Suzhou 215004, China

Corresponding author: TU Yi-Feng, tuyf@suda.edu.cn
Received Date: 1 October 2017
Accepted Date: 4 November 2017

Fund Project: This word was supported by the National Natural Science Foundation of China (No. 21375091)the National Natural Science Foundation of China 21375091

Figures(4)

In this work, a simple and fast approach for dipeptide detection was developed. AuNPs were decorated onto the surface of indium tin oxide (ITO) glass to serve as the working electrode to trigger the electrochemiluminescence (ECL) of luminol. The property of this electrode was characterized by transmission electron microscopy (TEM), scanning electron microscopy (SEM), electrochemistry and spectroscopic method. Under the optimum conditions, the dipeptide His-Ala could be detected within a linear range from 2.44×10^-11 mol/L to 1.22×10^-7 mol/L, with a detection limit of 2.42×10^-12 mol/L (S/N=3). In human body, the glucagon-like peptide 1(GLP-1) might lose activity during degradation under the enzymatic action of dipeptidyl peptidase Ⅳ (PDD-Ⅳ), meanwhile release same concentrated His-Ala dipeptide. Thus, the detection of His-Ala dipeptide was of great significance for investigation of diabetes not only for understanding the relation between GLP-1, PDD-Ⅳ and its inhibitor, but also the drug discovering because of its potential availability as the target to control the blood GLP-1 level of type Ⅱ diabetics.
- Electrochemiluminescence,
- Luminol,
- Diabetes,
- Glucagon-like peptide 1,
- Dipeptide,
- Dipeptidyl peptidase Ⅳ
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沈阳化工大学材料科学与工程学院沈阳 110142