Metabolomics Study on Susceptibility of Pneumonia in Children

LIN Li-Li ^1,2,3, ,
WANG Shou-Chuan ^1,2,3 ,
YANG Yan ⁴ ,
XIE Tong ^1,2 ,
ZHAO Xia ^1,2,3 ,
REN Jing ^1,2,3 ,
LI Wei-Wei ^1,2,3 ,
TAO Jia-Lei ^1,2,3 ,
FAN Si-Li ⁵ ,
SHAN Jin-Jun ^1,2

1.
Jiangsu Key Laboratory of Pediatric Respiratory Disease, Nanjing University of Chinese Medicine, Institute of Pediatrics, The First Clinical Medical College, Nanjing University of Chinese Medicine, Nanjing 210023, China;
2.
Medical Metabolomics Center, Nanjing 210023, China;
3.
The Affiliated Hospital of Nanjing University of Chinese Medicine, Nanjing 210029, China;
4.
Beijing Children's Hospital, Capital Medical University, Beijing 100045, China;
5.
Genome Center of UC Davis, NIH West Coast Metabolomics Center, Davis 95616, USA

Received Date: 12 September 2017
Revised Date: 22 December 2017

Fund Project: This work was supported by the National Natural Science Foundation of China (No.81373688), the Postgraduate Research and Innovation Project of Colleges and Universities of Jiangsu Province (No.SJLX_0432), Jiangsu Provincial 333 High-level Talents Cultivation Project (No.BRA2016427), and Jiangsu Provincial Six Talent Peaks Project (No.YY-022).

The changes of endogenous metabolites in urine samples that come from pneumonia patients of 6 months to 6 years old children were analyzed by metabolomics methods based on gas chromatography and mass spectrometry (GC-MS). The aim of this study was to analyze and study the pathogenesis of endogenous metabolites in children with pneumonia and the pathogenesis of pneumonia susceptibility. The urine samples were collected and divided into normal children group (NC group, n=29), first infection with pneumonia group (FIP group, n=35), and repeated infection with pneumonia group (RIP group, n=31). The urine metabolic profile of pneumonia was obtained by GC-MS. Principal component analysis (PCA) and partial least squares-discriminant analysis (PLS-DA) were used to analyze the data. The results were analyzed by one-way analysis of variance and Fold change. Finally, there was significant difference between the normal group and the pneumonia group, the significant metabolites were serine, histidine, proline, norleucine, glutamine, stearic acid, valine, isoleucine with p value <0.05 and Fold change>5, and indole-3-acetic acid, creatine, ethanolamine, mannosylglycerol and fructose were significant between the two pneumonia groups with p value <0.05. The urinary metabolites demonstrated that amino acid metabolism and glucose metabolism were the main metabolic pathways and responsible for the susceptibility to pneumonia.

Metabolomics Study on Susceptibility of Pneumonia in Children

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