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Citation: ZI Qin-Jiang, WANG Jin, CAO Qiu-E, ZHOU Chuan-Hua. Construction and Application of Target-induced Silver Deposition Based Colorimetric Assay[J]. Chinese Journal of Analytical Chemistry, ;2018, 46(8): 1215-1221. doi: 10.11895/j.issn.0253-3820.171157 shu

Construction and Application of Target-induced Silver Deposition Based Colorimetric Assay

1.
School of Chemical Science and Technology, Key Laboratory of Medicinal Chemistry for Natural Resource(Ministry of Education), Yunnan University, Kunming 650091, China;
2.
Eryuan Instritute for Food and Drug Control, Dali 671299, China

Received Date: 8 August 2017
Revised Date: 6 June 2018

Fund Project: This work was supported by the National Natural Science Foundation of China (Nos. 21505119, 21465025) and the Natural Science Foundation of Yunnan Province, China (No. 2015FD002).

Preparation of gold nanoparticles (AuNPs) with good stability is the key to construct metal nanoparticles based colorimetric assay method. Two kinds of AuNPs with glutathione as ligand were prepared using the one-step method and two-step method respectively. The particle size and colloid stability of the as-prepared AuNPs were compared. The two-step method prepared AuNPs was more stable. Then, a non-aggregation colorimetric strategy for detection of ascorbic acid (AA) based on metal nanoparticles was constructed using the AuNPs prepared by two-step method as seeds and catalyst. This strategy combined the target-induced silver deposition reaction and the extraordinarily high molar extinction coefficient of silver nanoparticles, which exhibited high detection sensitivity and good specificity. The presence of AA could reduce Ag⁺ to Ag0 on the surface of AuNPs, and the resulting color change of the detection solution and the A₄₁₀ was used for AA detection. The detection limit was as low as 0.75 μmol/L(S/N=3). In brief, a simple, fast and sensitive colorimetric assay method for detection of AA with good specificity and strong anti-interference ability was proposed. This method was applied to AA detection in real complex samples, which showed great prospect in bioanalytical applications.
- Gold nanoparticles,
- Ascorbic acid,
- Target-induced silver deposition,
- Colorimetric assay
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