Citation: HE Qiu-Ju, WANG Li-Qin. Hydrophobicity Analysis of Soy Protein in Soybean Water with Different Heating Temperatures during Painting and Calligraphy Restoration[J]. Chinese Journal of Analytical Chemistry, ;2018, 46(11): 1845-1850. doi: 10.11895/j.issn.0253-3820.171081 shu

Hydrophobicity Analysis of Soy Protein in Soybean Water with Different Heating Temperatures during Painting and Calligraphy Restoration

HE Qiu-Ju ^1,2, ,
WANG Li-Qin ¹

¹ Northwest University, Key Laboratory of Culture Heritage Research and Conservation, Ministry of Education, Xi'an 710069, China;
² Capital Museum, Key Laboratory of Beijing Cultural Relics and Museums Sci-tec Protection Research and Application, Beijing 100045, China

Received Date: 10 October 2017
Revised Date: 29 August 2018

Fund Project: This work was supported by the National Natural Science Foundation of China(No. 21175104), the Beijing Municipal Science and Technology Commission Project (No. Z161100002416020), and Beijing Outstanding Talent Cultivation Plan (No. 2014000020044).

Soybean water is a common sizing agent that is used in the restoration of Chinese paintings and calligraphies. Heating soybean water leads to denaturation of soy protein and thus alters the hydrophobicity of paper after sizing. In this work, the change of surface hydrophobicity of soybean protein in the heating process was analyzed by 8-aniline-1-naphthlene sulfonic acid (ANS) fluorescence probe. Sodium dodecyl sulfate polyacrylamide gel electrophoresis (SDS-PAGE) was adopted to sort out different protein contents which later were identified by LC-MS/MS. The self-deconvolution and second derivative curve fitting techniques of attenuated total reflectance-Fourier transform infrared spectroscopy (ATR-FTIR) were used to analyze the change of secondary structures of soybean protein at different heating temperatures. ANS fluorescence probe experiment showed that the soybean water achieved its largest hydrophobicity when the temperature was 80℃. The results of SDS-PAGE and LC-MS/MS revealed the disappearance of globulin glycinin G3 and glycoprotein lectin at high temperature. ATR-FTIR analysis showed that the increase of heating temperature of soybean water raised β-sheet content from 41.14% to 48.87% and the original α-helix, β-turn and γ-random structures shifted to β-sheet structure. There was a positive correlation between the structural content of β-sheet and the surface hydrophobicity. The research showed that the heating temperature of soybean milk at 80℃ was suitable for calligraphy and painting restoration.
- Soybean water,
- Heating temperature,
- Xuan paper,
- Soybean protein,
- Hydrophobicity
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