Citation: PENG Tao, WANG Jian-Yi, XIE San-Lei, YAO Kai, SUN Shu-Juan, ZENG Yu-Yang, JIANG Hai-Yang. Preparation of Protein Hybrid Fluorescence Nanoclusters for Rapid Detection of Mercury Ion[J]. Chinese Journal of Analytical Chemistry, ;2018, 46(3): 373-378. doi: 10.11895/j.issn.0253-3820.170353 shu

Preparation of Protein Hybrid Fluorescence Nanoclusters for Rapid Detection of Mercury Ion

College of Veterinary Medicine, China Agricultural University, Beijing 100193, China

Received Date: 5 June 2017
Revised Date: 19 January 2018

Fund Project: This work was supported by the National Natural Science Foundation (No. 31672600) and the National keypoint Research and Invention Program during the 13th Five-year Plan Period (No. 2017YFF0211003).

One-step green synthetic approach, with bovine serum albumin (BSA) as stabilizer and reductant, was developed for preparation of BSA hybrid fluorescence gold nanoclusters (AuNCs@BSA). The prepared AuNCs@BSA exhibited strong red fluorescence under UV light illumination. Upon excited at 360 nm, the fluorescence spectrum of AuNCs@BSA exhibited maximum emission peak at 635 nm. AuNCs@BSA was presented as uniform spherical morphology with diameter at (2.0±0.05) nm. The fluorescence of AuNCs@BSA could be quenched by Hg²⁺ because of its metallophilic reaction. Based on the fluorescent spectrometry, a rapid detection system was developed for Hg²⁺ detection in tap water. The AuNCs@BSA amount, pH and buffer system were optimized in this study. According to optimization results, ultrapure water (pH 5.0) was selected to dilute the AuNCs@BSA by 100 times, and 50 μL/well of AuNCs@BSA dilution was applied to detect mercury ion in tap water. Under the optimized conditions, the detection could be completed within 3 min, the fluorescence intensity of the system was linearly proportional to the concentration of mercury ion in the range of 0.5-900 μg/L with linear equations y=-26.76lgx + 803.1 (0.5-75 μg/L, R²=0.9951) and y=-0.27x+762.02 (75-900 μg/L, R²=0.9959). The limit of detection was 0.14 μg/L(3σ). The average recoveries in spiked tape water samples ranged from 86.8%-113.4% with relative standard deviation of less than 15%. The result implied that the developed method was able to apply to detect mercury ion rapidly, sensitively and conveniently.
- Bovine serum albumin,
- Fluorescence gold nanoclusters,
- Mercury ion,
- Tap water.
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