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Citation: DING Qiao-Qi, LI Li, FAN Wen-Tao, LYU Ya-Nan, HU Jian-Hua, YAN Li-Ping, SONG Su-Quan. Development of Quantum Dot Submicrobeads-based Fluorescent Immunochromatographic Test Strip for Rapid Detection of Chloramphenicol[J]. Chinese Journal of Analytical Chemistry, ;2017, 45(11): 1686-1693. doi: 10.11895/j.issn.0253-3820.170300 shu

Development of Quantum Dot Submicrobeads-based Fluorescent Immunochromatographic Test Strip for Rapid Detection of Chloramphenicol

College of Veterinary Medicine, Nanjing Agricultural University, Nanjing 210095, China

Received Date: 9 May 2017
Revised Date: 4 September 2017

Fund Project: This work was supported by the National Key R&D Program (Nos. 2016YFD0501200, 2016YFD0501009), the Fundamental Research Funds for the Central Universities (No. Y0201500195), the Natural Science Foundation of Jiangsu Province (No. BK20161452), the Key Program of Science and Technology Planning of Guangdong Province, China (No. 2017B020202010), and the Priority Academic Program Development of Jiangsu Higher Education Institutions (Jiangsu, China).

A fluorescent immunochromatographic test strip based on the quantum dots submicrobeads (QBs) was developed for quantitative detection of chloramphenicol (CAP). In this method, monoclonal antibody of CAP and OBs complex fluorescent probe was first prepared using 1-ethyl-3-(3-dimethylaminopropyl)carbodiimide/N-hydroxysuccinimide coupling approach, then complete antigen CAP-HS-BSA was synthesized and sprayed on nitrocellulose membrane as test line (T line). Similarly, goat anti-mouse antibody was sprayed as control line (C line). The time required for the analysis was 15 min, and the limit of detection (LOD) for CAP was 0.1 μg/L, with a working range of 0.1-100 μg/L. In spiked milk samples, the test strip demonstrated high recoveries in the range from 93.3% to 97.9% with relative standard deviations of less than 7%.
- Chloramphenicol,
- Quantum dot submicrobeads,
- Immunochromatographic test strip
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