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Citation: ZHAO Cheng-Jun, MA Xiong-Hui, Li Jian-Ping. An Insulin Molecularly Imprinted Electrochemical Sensor Based on Epitope Imprinting[J]. Chinese Journal of Analytical Chemistry, ;2017, 45(9): 1360-1366. doi: 10.11895/j.issn.0253-3820.170256 shu

An Insulin Molecularly Imprinted Electrochemical Sensor Based on Epitope Imprinting

Guangxi Key Laboratory of Electrochemical and Magnetochemical Function Materials, Guangxi Colleges and Universities Key Laboratory of Food Safety and Detection, College of Chemistry and Bioengineering, Guilin University of Technology, Guilin 541004, China

Received Date: 23 April 2017
Revised Date: 6 July 2017

Fund Project: This work was supported by the National Natural Science Foundation of China (Nos. 21375031, 21765006) and the Natural Science Foundation of Guangxi Province, China (No. 2015GXNSFFA139005).

A novel molecularly imprinted electrochemical sensor for direct detection of insulin was prepared based on epitope imprinting. C-Terminal polypeptide in insulin as template molecule was firstly self-assembled on the Au electrode. Then the molecularly imprinted polymer (MIP) was fabricated by electropolymerization with o-phenylenediamine (o-PD) as functional monomer on this Au electrode. After elution of template molecules by NaOH solution, the imprinting cavities were formed with the three-dimensional structure matched with the polypeptide in insulin molecules. The imprinting cavities could specifically recognize and rebind with insulin molecules. With K₃[Fe(CN)₆]/K₄[Fe(CN)₆] as a probe, the insulin was indirectly detected. There was a linear relationship between the response current and the insulin concentrations in the range of 1.0×10^-14-5.0×10^-13 mol/L, and the detection limit was 7.24×10^-15 mol/L. The developed sensor exhibited good selectivity and stability, and could be applied to the determination of serum samples.
- Insulin,
- Molecular imprinting,
- Electrochemical sensor,
- Epitope imprinting
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