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Citation: LI Tian-Tian, ZHANG Li-Yuan, YU Zhen-Long, MA Xiao-Chi, DENG Sa. Synthesis of Boronic Acid-functionalized Soluble Dendrimers and Its Application in Detection of Human Liver Microsomal Glycoprotein Based on Enzyme-linked Immunosorbent Assay[J]. Chinese Journal of Analytical Chemistry, ;2017, 45(9): 1259-1263. doi: 10.11895/j.issn.0253-3820.170251 shu

Synthesis of Boronic Acid-functionalized Soluble Dendrimers and Its Application in Detection of Human Liver Microsomal Glycoprotein Based on Enzyme-linked Immunosorbent Assay

1.
College of Pharmacy, Dalian Medical University, Dalian 116044, China;2 College of Basic Medical Sciences, Dalian Medical University, Dalian 116044, China

Received Date: 20 April 2017
Revised Date: 10 June 2017

Fund Project: This work was supported by the National Natural Science Foundation of China (No. 21505015), the Program of the Key Project in Liaoning Province Education Department, China (No. LZ2016001), the High Level Talent Innovation and Entrepreneurship Program of Dalian, China (No. 2016RQ041), the Natural Science Foundation of Liaoning Province, China (No. 20170540286) and the Program of China Scholarships Council(No. 201608210312)

The glycoproteins in the biological sample are low abundance and are susceptible to be inhibited and interfered by other non-glycoproteins. An enrichment step is usually required before the glycoprotein analysis, but the operation steps of conventional solid-phase-based glycoprotein enrichment methods are difficult to be compatible with the most classical enzyme-linked immune sorbent assay (ELISA) technique. In this study, a novel water-soluble dendrimer based boronic acid capture (DBC) material was developed using PAMAM 4.0 as the carrier and boronic acid as the affinity group. The method was applied to the detection of glycoproteins in human liver microsomes using ELISA. In this study, the DBC enrichment conditions were optimized by model glycoprotein, and then its sensitivity and anti-interference ability were investigated. This method was applied to the enrichment of glycoproteinsin human liver microsomal. The results showed that the enrichment selectivity of DBC for glycoprotein could be up to 100000 folds, and the enrichment signal of glycoprotein could be increased by 100 times. Therefore, the ELISA method using DBC as a novel enrichment material for glycoprotein had high sensitivity and selectivity in detection of biological samples with only one simple incubation step, which was useful for glycoproteins researches.
- Glycoprotein,
- Boronic acid,
- Dendrimer,
- Enrichment
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Synthesis of Boronic Acid-functionalized Soluble Dendrimers and Its Application in Detection of Human Liver Microsomal Glycoprotein Based on Enzyme-linked Immunosorbent Assay

南开大学师唯/华北电力大学（保定）刘景维：二维配位聚合物中有序的亲锂冠醚位点用于无枝晶锂沉积

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通讯作者: 陈斌, bchen63@163.com