Citation: HAN Jiao-Jiao, HU Li-Ming, YI Yang, LIU Miao, XIA Jun, XU Guo-Mao, LUO Kai, WANG Qi, LAI Wei-Hua. Development of Colloidal Gold Lateral Flow Immunoassay for Quantitative Detection of Florfenicol[J]. Chinese Journal of Analytical Chemistry, ;2017, 45(8): 1188-1194. doi: 10.11895/j.issn.0253-3820.170207 shu

Development of Colloidal Gold Lateral Flow Immunoassay for Quantitative Detection of Florfenicol

HAN Jiao-Jiao ^1, ,
HU Li-Ming ¹ ,
YI Yang ¹ ,
LIU Miao ¹ ,
XIA Jun ² ,
XU Guo-Mao ² ,
LUO Kai ¹ ,
WANG Qi ¹ ,
LAI Wei-Hua ¹

1.
State Key Laboratory of Food Science and Technology, Nanchang 330047, China;2 Jiangxi Institute of Veterinary Drug and Feedstuff Control, Nanchang 330047, China

Received Date: 31 March 2017
Revised Date: 19 May 2017

A method for quantitative detection of florfenicol by colloidal gold lateral flow immunoassay was developed. The experimental conditions including pH value, concentrations of antibody in the process of conjugation between the colloidal gold and antibody, amount of gold-labeled antibody, concentration of the antigen sprayed on test lines (T line), and detection time were optimized. With a colloidal gold strip reader, the signal intensity of T lines and control lines (C line) on lateral flow strips was recorded. The T/C ratio of negative control and positive samples was defined as B₀ and B_x, and the standard curve was established by plotting the B_x/B₀ ratio against the concentration of florfenicol. This assay showed a good linear range from 0.1 to 1.5 ng/mL with the limit of detection of 0.08 ng/mL, while the result could be obtained within 15 min. The result showed that this quantitative method was convenient and rapid, and could be used in screening a large amount of samples on site.
- Colloidal gold,
- Florfenicol,
- Lateral flow immunoassay,
- Quantitative detection,
- Strip
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