Citation: LIU Jun-Shan, ZHANG Yang-Yang, WANG Zhong, DENG Jia-Yi, YE Xuan, XUE Ri-Ye, GE Dan, XU Zheng. Design and Validation of a Microfluidic Chip with Micropillar Arrays for Three-dimensional Cell Culture[J]. Chinese Journal of Analytical Chemistry, ;2017, 45(8): 1109-1115. doi: 10.11895/j.issn.0253-3820.170198 shu

Design and Validation of a Microfluidic Chip with Micropillar Arrays for Three-dimensional Cell Culture

LIU Jun-Shan ^1, ,
ZHANG Yang-Yang ¹ ,
WANG Zhong ¹ ,
DENG Jia-Yi ² ,
YE Xuan ² ,
XUE Ri-Ye ³ ,
GE Dan ² ,
XU Zheng ¹

1.
Key Laboratory for Micro/Nano Technology and System of Liaoning Province, Dalian University of Technology, Dalian 116024, China;2 College of Chemistry, Dalian University of Technology, Dalian 116024, China;3 Department of Engineering Mechanics, Dalian University of Technology, Dalian 116024, China

Received Date: 28 March 2017
Revised Date: 19 April 2017

Fund Project: This work was supported by the National Natural Science Foundation of China (Nos.51475080,51621064,31300809),the National Key Research and Development Program (No.2016YFC1202503),the National Science and Technology Support Program (No.2015BAI03B08),and the Research Funds for the Central Universities (Nos.DUT16QY06,DUT16TD20)

A microfluidic chip with micropillar arrays for three-dimensional (3D) cell culture was designed and validated. The chip consisted of a polydimethylsiloxane (PDMS) channel plate and a glass cover plate. One cell culture chamber composed of two rows of micropillar arrays and two lateral channels for transporting the culture medium were integrated on the PDMS channel plate. The spacing between micropillars directly affects the chip performance, which is critical for the design of the chip. In this work, the spacing between micropillars was optimized by numerical simulation and experimental validation. With the optimized microfluidic chip, the mixture of cells and extracellular matrix mimics could be steadily injected into the cell culture chamber, the nutrients in the culture medium from the lateral channels could quickly diffuse into the chamber, and the cell metabolites could also timely diffuse out of the chamber. To test the stability of the microenvironment in the microfluidic chip, neural stem cells were three-dimensionally cultured.
- Microfluidic chip,
- Cell,
- Three-dimensional culture,
- Micropillar,
- Polydimethylsiloxane
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