Citation: WU Dan, YANG Jun-Jun, YANG Fan, ZHANG Bo-Yong, DU Jie, WANG Yue-Fei, XU Rui-Qing. Analysis of Alkaline and Neutral Volatile Metabolites in Feces by Gas Chromatography-Tandem Mass Spectrometry[J]. Chinese Journal of Analytical Chemistry, ;2017, 45(6): 837-843. doi: 10.11895/j.issn.0253-3820.170184 shu

Analysis of Alkaline and Neutral Volatile Metabolites in Feces by Gas Chromatography-Tandem Mass Spectrometry

WU Dan ^1,2, ,
YANG Jun-Jun ^1,2 ,
YANG Fan ^1,2 ,
ZHANG Bo-Yong ^1,2 ,
DU Jie ^1,2 ,
WANG Yue-Fei ^1,2 ,
XU Rui-Qing ³

1.
Tianjin State Key Laboratory of Modern Chinese Medicine, Tianjin University of Traditional Chinese Medicine, Tianjin 300193, China;
2.
Research and Development Center of Traditional Chinese Medicine, Tianjin International Joint Academy of Biotechnology and Medicine, Tianjin 300457, China;
3.
(Center for Disease Control and Prevention of China National Offshore Oil Corporation Ltd. , Tianjin 300452, China

Received Date: 13 January 2017
Revised Date: 10 April 2017

Fund Project: This work was supported by the National Science and Technology Major Projects for "Major New Drugs Innovation and Development" (No. 2015ZX09J15102-004-004) and the Tianjin Support Plans for the Top of the Notch Youth Talents.

A rapid gas chromatography-tandem mass spectrometric (GC-MS) method was developed for the analysis of alkaline and neutral volatile metabolites of human and rat feces. Feces were extracted by 75% (V/V) methanol solution. After addition of ammonia solution (pH 10, final concentration of 1%), the supernatant was subjected to GC-MS analysis. In the experiment, a CP-Sil 5 (25 m×0.25 mm×0.12 μm) capillary column was utilized to separate the interesting compounds. Helium was employed as a carrier gas at a constant flow rate of 1 mL/min. Oven temperature was programmed as follows:the initial temperature was set at 50℃ and held for 1 min, then increased at 25℃/min to 150℃, sustained for 1 min; increased at 20℃/min to 200℃, held for 2 min; and finally increased at 10℃/min to 270℃, maintained for 5 min. The MS was operated in the electron impact ionization mode at -70 eV. The injector, ion source and transfer line temperatures were maintained at 220℃, 230℃ and 280℃, respectively. Mass data were acquired in full scan mode from m/z 10 to 600. The solvent delay time was set for 3 min. A total of 11 volatile components were identified in the feces from human and 7 in the feces from rats by retrieving the NIST library, comparing with the standards and analyzing the MS data. The method was simple and sensitive in the detection of alkaline and neutral volatile metabolites of feces from human and rats.
- Gas chromatography-tandem mass spectrometry,
- Feces,
- Gut microbiota,
- Alkaline metabolites
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