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Citation: GU Qiao-Rong, AI Jun-Jie, ZHANG Qian-Yun, DONG Ya-Nan, GAO Qiang. Fluorescence Assay for Phospholipase C Activity Using Liposome Probes[J]. Chinese Journal of Analytical Chemistry, ;2017, 45(9): 1278-1283. doi: 10.11895/j.issn.0253-3820.170179 shu

Fluorescence Assay for Phospholipase C Activity Using Liposome Probes

School of Chemistry and Chemical Engineering, Shaanxi Normal University, Xi'an 710062, China

Received Date: 23 March 2017
Revised Date: 18 July 2017

Fund Project: This work was supported by the National Natural Science Foundation of China (No.21175089).

A simple assay for detection of phospholipase C (PLC) activity was developed based on a fluorescence liposome probe using the Liss Rhod PE-loaded phospholipid liposomes. The liposome probe was prepared by the coassembly of 1,2-dipalmitoyl-sn-glycero-3-phosphocholine (DPPC) and fluorescent lipid (Liss Rhod PE). The probe showed very low background fluorescence due to fluorescence self-quenching effect of Liss Rhod PE. As the PLC enzyme selectively digested lipid, the Rhod fluorescence was recovered from its quenched state, leading to the sensitive detection of PLC. This assay provided a limit of detection (at a signal-to-noise ratio of 3) of 2 U/L for PLC. In the presence of PLC inhibitor, the fluorescent response of the sensor for PLC decreased, indicating that the assay could also be used for screening PLC inhibitors.
- Fluorescence,
- Liposome,
- Probe,
- Phospholipase C,
- Inhibitor
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