Citation: CHEN Bin, WANG Shi-Hui, LI Rong, FAN An. Study on Binding Characteristics of Aminocarboxyl Chelating Ligands for Metal Ions[J]. Chinese Journal of Analytical Chemistry, ;2017, 45(9): 1303-1308. doi: 10.11895/j.issn.0253-3820.170154 shu

Study on Binding Characteristics of Aminocarboxyl Chelating Ligands for Metal Ions

School of Chemical Engineering, Northwest University, Xi'an 710069, China

Received Date: 15 March 2017
Revised Date: 22 June 2017

Fund Project: This work was supported by the National Natural Science Foundation of China (No.21376191) and the Natural Science Foundation of Shaanxi Province, China (No. 2011JM2013), the Service Local Special Project of Shaanxi Province Education Department (No.14JF027) and the Industrialization Cultivation Item of Shaanxi Province Educational Department (No.2013jc23)

The feasibility of frontal chromatography for determining the complexation stability constant K_ML and total mole of binding site Λ_o was demonstrated by the accuracy and precision binding experiments between metal ions (Cu²⁺, Ni²⁺ and Co²⁺) and chelating ligand (IDA), in which R² > 0.98 and RSD <5%. To further prove the universality of the frontal chromatography, the changing rules of K_ML values between Cu²⁺, Ni²⁺, Co²⁺ and IDA, Asp, L-Glu were examined under NaAc-HAc, Na-PB and Tris-HCl buffer systems. The results showed that the binding strength of chelating ligands for metal ions followed IDA > Asp > Glu; binding strength of metal ions for chelate ligands followed Cu²⁺>Ni²⁺>Co²⁺; and the binding effect with NaAc-HAc buffer was the best. In aqueous solution, quantum computing of binding energy (ΔE) and gibbs free energy (ΔG) between chelating ligand and metal ion was performed at the M06/6-311++G (d, p) level. According to ΔE and ΔG, the binding rules between chelating ligand and metal ion were predicted theoretically. These rules were basically in agreement with above experimental results. The present work provided effective method for studying on binding characteristics of metal ions for aminocarboxyl chelating ligands, thus exhibited a good foundation for improving the stability of immobilized metal affinity chromatographic column and solving the leakage of metal ions from the column in the process of competitive elution.
- Frontal chromatography,
- Quantum computing,
- Aminocarboxyl chelating ligand,
- Complexation stability constant,
- Total mole of binding site
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