Citation: HU Xing-Jie, WANG Ze-Jun, YIN Min, LI Di, CHEN Nan. Effect of Serum on Cellular Uptake of Tetrahedral Deoxyribonucleic Acid Nanostructure[J]. Chinese Journal of Analytical Chemistry, ;2017, 45(7): 951-957. doi: 10.11895/j.issn.0253-3820.170104 shu

Effect of Serum on Cellular Uptake of Tetrahedral Deoxyribonucleic Acid Nanostructure

HU Xing-Jie ^1,2, ,
WANG Ze-Jun ^1,2 ,
YIN Min ¹ ,
LI Di ¹ ,
CHEN Nan ¹

1.
Division of Physical Biology & Bioimaging Center, Shanghai Synchrotron Radiation Facility, Shanghai, Institute of Applied Physics, Chinese Academy of Sciences, Shanghai 201800, China;
2.
University of Chinese Academy of Sciences, Beijing 100049, China

Received Date: 21 February 2017
Revised Date: 21 April 2017

Fund Project: This work was supported by the National Narural Science Foundation of China (Nos. 31470970, 21675166 and 21505148 ) and the Youth Innovation Promotion Association, Chinese Academy of Sciences (No. 2015211)

In the present study, the effect of serum (fetal bovine serum, FBS) on cellular uptake behaviors of DNA tetrahedron (TDNs) was investigated. Fluorescence-labeled TDNs were synthesized by self-assembly and purified with HPLC to achieve TDNs product with purity of 95%. By means of flow cytometry and confocal microscopy, the kinetics of TDNs endocytosis in HeLa cells in the presence or absence of FBS was compared. The results showed that TDNs remained intact in complete medium and cell lysate for more than 12 hours. FBS increased the amount of internalized TDNs in HeLa cells, whereas it did not change the route of caveolin-dependent endocytosis. Herein, our study provided a new insight into the effects of biomolecules on the interaction between cells and DNA nanostructures, which helped the future development of DNA-based intracellular nanocarriers.
- Deoxyribonucleic acid nanostructure,
- Deoxyribonucleic acid tetrahedron,
- Cellular uptake,
- Serum,
- Endocytosis
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