Citation: WANG Xue, LI Peng-Jun, QIU Ping, WANG Xiao-Lei. A Highly Sensitive Sandwich Immunosensor for Determination of α-Fetoprotein[J]. Chinese Journal of Analytical Chemistry, ;2017, 45(8): 1180-1187. doi: 10.11895/j.issn.0253-3820.170019 shu

A Highly Sensitive Sandwich Immunosensor for Determination of α-Fetoprotein

1.
Department of Chemistry, Nanchang University, Nanchang 330031, China;2 Institute of Translational Medicine, Nanchang University, Nanchang 330088, China

Received Date: 11 January 2017
Revised Date: 8 June 2017

Fund Project: This work was supported by the National Natural Science Foundation of China (No.21461015).

The Au-PDA-Fe3O4 decorated graphene oxide electrode was modified with Prussion blue-secondary antibodies for determination of cancer biomarker α-fetoprotein (AFP) with great sensitivity. Scanning electron microscopy (SEM), X-ray diffraction (XRD) and ultraviolet-visible absorption spectrometry were used to characterize the structure of the material. Greatly enhanced sensitivity for the cancer biomarker is based on a dual signal amplification strategy. First, Fe₃O₄-PDA-Au used for the biosensor platform increased the surface area to capture a large amount of primary antibodies (Ab1), which resulted in amplification of the detection response. Second, graphene oxide allowed several binding events of PB-Ab2. Enhanced sensitivity was also achieved by introducing the multibioconjugates of Ab2-PB-GO onto the electrode surface through "sandwich" immunoreactions. The test result shows that there are linear relationships between current and AFP concentrations ranging from 0.005 ng/mL to 1 ng/mL and 1 ng/mL to 20 ng/mL, with the low detection limit (LOD) of 1.0 pg/mL. This immunosensor is simple, low-cost and sensitive and shows a great potential in biomedicine, clinical diagnosis and health examination.
- Gold nanoparticles,
- Graphene oxide,
- α-Fetoprotein,
- Prussian blue,
- Sandwich immunosensor
1. [1]
2. [2]
3. [3]
4. [4]
5. [5]
6. [6]
7. [7]
8. [8]
9. [9]
10. [10]
11. [11]
12. [12]
13. [13]
14. [14]
15. [15]
16. [16]
17. [17]
18. [18]
19. [19]
20. [20]
21. [21]
22. [22]
23. [23]
24. [24]
25. [25]
26. [26]
27. [27]
28. [28]
29. [29]
30. [30]
31. [31]
32. [32]
33. [33]
34. [34]
35. [35]
36. [36]
37. [37]
38. [38]
39. [39]
40. [40]
1. [1]
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1.
沈阳化工大学材料科学与工程学院沈阳 110142