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Citation: ZHAO Bing-Jie, ZHAO Jin-Bao, QI Xiao-Hua, ZOU Ming-Qiang, CHEN Yan, ZHANG Fan, YANG Yi. Development of Immunochromatographic Strips Based on Covalently Conjugated BHHCT-Eu³⁺@SiO₂ for Rapid and Quantitative Detection of Kanamycin[J]. Chinese Journal of Analytical Chemistry, ;2017, 45(10): 1467-1474. doi: 10.11895/j.issn.0253-3820.170015 shu

Development of Immunochromatographic Strips Based on Covalently Conjugated BHHCT-Eu³⁺@SiO₂ for Rapid and Quantitative Detection of Kanamycin

1.
College of Science, Beijing University of Chemical Technology, Beijing, 100029, China;2 Chinese Academy of Inspection and Quarantine, Beijing 100123, China

Received Date: 10 January 2017
Revised Date: 28 August 2017

Fund Project: This work was supported by the Key Projects in the National Science & Technology Pillar Program during the Twelfth Five-year Plan Period, China (No. 2015BAK44B01) and the National Natural Science Foundation of China (No.21675008).

As a kind of fluorescent nanoprobe, BHHCT-Eu³⁺@SiO₂ fluorescent nanoparticles were synthesized using microwave irradiation. Transmission electron microscopy characterization showed that the nanoparticles were in spherical shape with particle size of about 36 nm. The BHHCT-Eu³⁺@SiO₂ exhibited good fluorescence property, with a maximal excitation wavelength of 343 nm and an maximal emission wavelength of 615 nm. The fluorescence lateral flow immunoassay (LFIA) was established for rapid and quantitative detection of kanamycin (Kana) in milk after BHHCT-Eu³⁺@SiO₂ fluorescent nanoparticles were conjugated with Kana antibody, with dextran as a linker. The limit of detection of Kana with the LFIA method was 0.85 ng/mL with IC₅₀ of 12.76 ng/mL, and the detection range was from 3.0 ng/m to 76 ng/mL. The recoveries of Kana in milk were between 93.7% and 97.4% with RSD of 3.1%-4.6%, and cross-reactivity of the fluorescence immunoassay strip for Kana determination was < 1%. The detection results of kana in milk samples between the LFIA and traditional ELISA method showed good correlation.
- Kanamycin,
- Microwave-assisted,
- Europium chelate-bonded silica nanoparticles,
- Fluorescence lateral flow immunoassay,
- Milk
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Development of Immunochromatographic Strips Based on Covalently Conjugated BHHCT-Eu3+@SiO2 for Rapid and Quantitative Detection of Kanamycin

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通讯作者: 陈斌, bchen63@163.com

Development of Immunochromatographic Strips Based on Covalently Conjugated BHHCT-Eu³⁺@SiO₂ for Rapid and Quantitative Detection of Kanamycin