Citation: LIU Zhen-Jie, SHI Zhi-Long, WANG Jia-Bo, ZHANG Hai-Zhu, LI Chun-Yu, NIU Ming, HE Qin, ZHEN Han-Shen, XIAO Xiao-He. Quality Evaluation of Salvia Miltiorrhiza Bge. Based on Chemical Profile and Bioassay of Anti-platelet Aggregation[J]. Chinese Journal of Analytical Chemistry, ;2017, 45(5): 693-699. doi: 10.11895/j.issn.0253-3820.160864 shu

Quality Evaluation of Salvia Miltiorrhiza Bge. Based on Chemical Profile and Bioassay of Anti-platelet Aggregation

1.
China Military Institute of Chinese Medicine, 302 Military Hospital, Beijing 100039, China;
2.
Guangxi Botanical Garden of Medicinal Plants, Nanning 530023, China;
3.
State Key Laboratory Breeding Base of Systematic Research, Development and Utilization of Chinese Medicine Resources, Chengdu University of Traditional Chinese Medicine, Chengdu 610075, China;
4.
College of Pharmacy, Guangxi University of Traditional Chinese Medicine, Nanning 530001, China

Received Date: 26 November 2016
Revised Date: 3 March 2017

Fund Project: This work was supported by the National Natural Science Foundation of China (No. 81274026), the National Industry Science & Technology Foundation (No.201507002), the Open Research Fund of the State Key Laboratory Breeding Base of Systematic Research, and the Development and Utilization of Chinese Medicinal Resources and the Key Army Project (No.BWS11J049).

Chemical analysis and bioassay were used to distinguish quality grades of Salvia miltiorrhiza bge., and the active compounds were investigated on anti-platelet aggregation. We established the HPLC fingerprint of Salvia miltiorrhiza bge. and meansured thir biopotency based on anti-platelet aggregation. The results showed that the HPLC fingerprints of different batches of Salvia miltiorrhiza bge. were similar (Similarity=0.930-0.998), but the difference of biopotency could be ten-fold. Through correlation analysis of chemical fingerprint and anti-platelet aggregation assay, dihydrotanshinone Ⅰ, cryptotanshinone, tanshinone Ⅰ, tanshinone ⅡA, and two unknown compounds were closely related to the biopotency. It was proved that cryptotanshinone was a high activity component on platelet aggregation. To compare the active contribution of the high content Salvianolic acid B and low content of the active ingredient cryptotanshinone, the active contribution value of the two compounds were basically equivalent. Cryptotanshinone, which had low content but high activity, made an important contribution to evaluating the quality of Salvia miltiorrhiza bge.. The results indicated that the quality of traditional Chinese medicine could not be evaluated accurately by only using the chemical content or abundance. This work established a quality evaluation model based on chemical fingerprint and bioassay which could improve the quality evaluation of traditional Chinese medicine associating with effects.
- Salvia miltiorrhiza bge.,
- Anti-platelet aggregation,
- Chemical fingerprint,
- Bioassay,
- Spectrum-effect relationship,
- Quality evaluation
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沈阳化工大学材料科学与工程学院沈阳 110142