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Citation: SONG Ai-Ying, WANG Li-Hong, ZHANG Yan-Xia, SHI En-Lin, ZENG Jun-Ling. Determination of Caffeine in Urea by Bubble-in-drop Single Drop Microextraction and Gas Chromatography-Mass Spectrometry[J]. Chinese Journal of Analytical Chemistry, ;2017, 45(5): 707-712. doi: 10.11895/j.issn.0253-3820.160862 shu

Determination of Caffeine in Urea by Bubble-in-drop Single Drop Microextraction and Gas Chromatography-Mass Spectrometry

1.
School of Public Security & Technology, Gansu Institute of Political Science and Law, Lanzhou 730070, China;
2.
Practice and Training Bases for New Agricultural Technology, Lanzhou Military Area Command of Chinese People's Liberation Army, Baiyin 730617, China

Received Date: 24 November 2016
Revised Date: 15 March 2017

Fund Project: This work was supported by the National Natural Science Foundation of China (No. 21565005), the Natural Science Foundation of Gansu Province, China (No. 1506RJZA033) and the Project on Science and Technology of Lanzhou City (No. 2016-3-36)

A method for the determination of caffeine in urea was developed based on bubble-in-drop single drop microextraction (BID-SDME) followed by gas chromatography/mass spectrometry (GC-MS). Under the optimum conditions including chloroform as extraction solvent, an exposure volume of 1 μL, a bubble volume of 1.6 μL, stirring for 5 min at 300 r/min, 15% (m/V) NaCl, and a distance of 1 cm between bubble and stirring bar, the detection limit of this method was as low as 0.003 mg/L and the linear range was from 0.005 mg/L to 10 mg/L with correlation coefficient of 0.982. The recoveries of caffeine were from 89.2% to 107.5% at different spiked levels in human urine and the relative standard deviation (RSD, n=6) was less than 8%.
- Bubble-in-drop single drop microextraction,
- Caffeine,
- Gas chromatography-mass spectrometry,
- Urea
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