Home

Citation: LIU Zhuo, LI Hong-Jun, LI Xiao-Ou, YANG Zhao-Wei, ZHAO Li-Chun, HUANG Li-Hong, GUO Hong-Hua, WANG Hai, ZHAO Qing, HE Cheng-Yan. Differential Proteomics Analysis of Tumor Tissues and Their Adjacent Normal Tissues from Patients with Colorectal Cancer by Gel Electrophoresis-Capillary Liquid Chromatography-Mass Spectrometry-based Approach[J]. Chinese Journal of Analytical Chemistry, ;2017, 45(3): 322-330. doi: 10.11895/j.issn.0253-3820.160851 shu

Differential Proteomics Analysis of Tumor Tissues and Their Adjacent Normal Tissues from Patients with Colorectal Cancer by Gel Electrophoresis-Capillary Liquid Chromatography-Mass Spectrometry-based Approach

1.
China-Japan Union Hospital, Jilin University, Changchun 130033, China;
2.
Tumor Hospital of Jilin Province, Changchun 130021, China;
3.
College of Pharmacology, Jilin University, Changchun 130023, China

Received Date: 22 November 2016
Revised Date: 13 January 2017

Fund Project: This work was supported by the National Natural Science Foundation of China (Nos. 81572082, 81472030) and Jilin Province Science and Technology Deparement, China (Nos. 2011713, 20150414015GH, 20150101153JC, 20140311092YY, 2014041005GH)

The whole protein extracts from tissue specimens (tumor and noncancerous) from patients with colorectal cancer were divided into several fractions with different molecular weight by gel electrophoresis, which were then subject to in-gel digestion and proteomics analysis by capillary liquid chromatography-mass spectrometry. A total of 29 differentially expressed proteins were identified, among which 19 were up-regulated proteins in tumor tissues, such as Fibrinogen beta chain, Vimentin, Fibrinogen gamma chain, Histone H2A type 1-B/E, Isoform 1 of Periostin, Fibrinogen alpha chain, Histone H3.1, Alpha-enolase, Protein disulfide-isomerase A3, and so on. Ten proteins were found to be down-regulated, including Sarcomeric tropomyosin kappa, Transgelin, Heat shock protein beta-1, Talin-1, Isoform 2 of Vinculin, Isoform 1 of Filamin-C, and so on. The differential expressions of Talin-1 and Alpha-enolase were selected to be verified by Western blot analysis. Bioinformatics analysis results indicated that most of the up-regulated proteins in colorectal cancer tissues had the feature of protein exocytosis, suggesting that these proteins were likely to penetrate into the circulatory system in the body, which could be detected in blood or ascites. On the other hand, most of the down-regulated proteins belonged to the cytoskeleton and extracellular matrix fibers, which were closely related to the invasion and metastasis of tumor cells into their surrounding tissues. This study provided basic data for the early diagnosis of colorectal cancer, colorectal cancer screening and other aspects, which had important significance to further study the pathogenesis of colorectal cancer.
- Gel electrophoresis-capillary liquid chromatography-mass spectrometry,
- Colorectal cancer,
- Proteome,
- Talin 1,
- Enolase 1
1. [1]
2. [2]
3. [3]
4. [4]
5. [5]
6. [6]
7. [7]
8. [8]
9. [9]
10. [10]
11. [11]
12. [12]
13. [13]
14. [14]
15. [15]
16. [16]
17. [17]
18. [18]
1. [1]
  Xinran Zhang , Siqi Liu , Yichi Chen , Qingli Zou , Qinghong Xu , Yaqin Huang . From Protein to Energy Storage Materials: Edible Gelatin Jelly Electrolyte. University Chemistry, 2025, 40(7): 255-266. doi: 10.12461/PKU.DXHX202408104
2. [2]
  Xinyi Hong , Tailing Xue , Zhou Xu , Enrong Xie , Mingkai Wu , Qingqing Wang , Lina Wu . Non-Site-Specific Fluorescent Labeling of Proteins as a Chemical Biology Experiment. University Chemistry, 2024, 39(4): 351-360. doi: 10.3866/PKU.DXHX202310010
3. [3]
  Zeyu XU , Anlei DANG , Bihua DENG , Xiaoxin ZUO , Yu LU , Ping YANG , Wenzhu YIN . Evaluation of the efficacy of graphene oxide quantum dots as an ovalbumin delivery platform and adjuvant for immune enhancement. Chinese Journal of Inorganic Chemistry, 2024, 40(6): 1065-1078. doi: 10.11862/CJIC.20240099
4. [4]
  Zhuoming Liang , Ming Chen , Zhiwen Zheng , Kai Chen . Multidimensional Studies on Ketone-Enol Tautomerism of 1,3-Diketones By ¹H NMR. University Chemistry, 2024, 39(7): 361-367. doi: 10.3866/PKU.DXHX202311029
5. [5]
  Yongjie ZHANG , Bintong HUANG , Yueming ZHAI . Research progress of formation mechanism and characterization techniques of protein corona on the surface of nanoparticles. Chinese Journal of Inorganic Chemistry, 2024, 40(12): 2318-2334. doi: 10.11862/CJIC.20240247
6. [6]
  Yihui Song , Shangshang Qin , Kai Wu , Chengyun Jin , Bin Yu . 生物化学在高水平创新型药学人才培养中的交叉融合应用——以去甲基化酶LSD1抑制剂的活性评价为例. University Chemistry, 2025, 40(6): 341-352. doi: 10.12461/PKU.DXHX202406018
7. [7]
  Yujing Chen , Hongqun Ouyang , Dan Zhao , Yanyan Chu , Zhengping Qiao . Recommendations for the Content and Instruction of the Physical Chemistry Experiment “Construction of Ternary Liquid-Liquid Phase Diagrams”. University Chemistry, 2025, 40(7): 359-366. doi: 10.12461/PKU.DXHX202409120
8. [8]
  Wujun Jian , Mong-Feng Chiou , Yajun Li , Hongli Bao , Song Yang . Cu-catalyzed regioselective diborylation of 1,3-enynes for the efficient synthesis of 1,4-diborylated allenes. Chinese Chemical Letters, 2024, 35(5): 108980-. doi: 10.1016/j.cclet.2023.108980
9. [9]
  Chunhua Ma , Mengjiao Liu , Siyu Ouyang , Zhenwei Cui , Jingjing Bi , Yuqin Jiang , Zhiguo Zhang . Metal-free construction of diverse 1,2,4-triazolo[1,5-a]pyridines on water. Chinese Chemical Letters, 2025, 36(1): 109755-. doi: 10.1016/j.cclet.2024.109755
10. [10]
  Liangfeng Yang , Liang Zeng , Yanping Zhu , Qiuan Wang , Jinheng Li . Copper-catalyzed photoredox 1,4-amidocyanation of 1,3-enynes with N-amidopyridin-1-ium salts and TMSCN: Facile access to α-amido allenyl nitriles. Chinese Chemical Letters, 2024, 35(11): 109685-. doi: 10.1016/j.cclet.2024.109685
11. [11]
  . 第41卷第1期封面和目次. Acta Physico-Chimica Sinica, 2025, 41(1): -.
12. [12]
  Huiju Cao , Lei Shi . sp¹-Hybridized linear and cyclic carbon chain. Chinese Chemical Letters, 2025, 36(4): 110466-. doi: 10.1016/j.cclet.2024.110466
13. [13]
  Huijuan Liao , Yulin Xiao , Dong Xue , Mingyu Yang , Jianyang Dong . Synthesis of 1-Benzyl Isoquinoline via the Minisci Reaction. University Chemistry, 2025, 40(7): 294-299. doi: 10.12461/PKU.DXHX202409092
14. [14]
  . Cover and Table of Contents for Vol.41 No. 12. Acta Physico-Chimica Sinica, 2026, 42(1): 0-.
15. [15]
  Gaofeng WANG , Shuwen SUN , Yanfei ZHAO , Lixin MENG , Bohui WEI . Structural diversity and luminescence properties of three zinc coordination polymers based on bis(4-(1H-imidazol-1-yl)phenyl)methanone. Chinese Journal of Inorganic Chemistry, 2024, 40(5): 849-856. doi: 10.11862/CJIC.20230479
16. [16]
  Hai-Yang Song , Jun Jiang , Yu-Hang Song , Min-Hang Zhou , Chao Wu , Xiang Chen , Wei-Min He . Supporting-electrolyte-free electrochemical [2 + 2 + 1] annulation of benzo[d]isothiazole 1,1-dioxides, N-arylglycines and paraformaldehyde. Chinese Chemical Letters, 2024, 35(6): 109246-. doi: 10.1016/j.cclet.2023.109246
17. [17]
  Zhiwei Chen , Heyun Sheng , Xue Li , Menghan Chen , Xin Li , Qiuling Song . Efficient capture of difluorocarbene by pyridinium 1,4-zwitterionic thiolates: A concise synthesis of difluoromethylene-containing 1,4-thiazine derivatives. Chinese Chemical Letters, 2024, 35(4): 108937-. doi: 10.1016/j.cclet.2023.108937
18. [18]
  Jinge Zhu , Ailing Tang , Leyi Tang , Peiqing Cong , Chao Li , Qing Guo , Zongtao Wang , Xiaoru Xu , Jiang Wu , Erjun Zhou . Chlorination of benzyl group on the terminal unit of A₂-A₁-D-A₁-A₂ type nonfullerene acceptor for high-voltage organic solar cells. Chinese Chemical Letters, 2025, 36(1): 110233-. doi: 10.1016/j.cclet.2024.110233
19. [19]
  Qi Li , Zi-Lu Wang , Yun-He Xu . Copper-catalyzed 1,4-silylcyanation of 1,3-enynes: A silyl radical-initiated approach for synthesis of difunctionalized allenes. Chinese Chemical Letters, 2025, 36(3): 109991-. doi: 10.1016/j.cclet.2024.109991
20. [20]
  Yuanyuan Ping , Wangqing Kong . 光催化碳氢键官能团化合成1-苯基-1,2-乙二醇. University Chemistry, 2025, 40(6): 238-247. doi: 10.12461/PKU.DXHX202408092

Get Citation

PDF

XML

Metrics

PDF Downloads(2)
Abstract views(575)
HTML views(57)

通讯作者: 陈斌, bchen63@163.com

1.
沈阳化工大学材料科学与工程学院沈阳 110142

Address：Zhongguancun North First Street 2,100190 Beijing, PR China Tel: +86-010-82449177-888

DownLoad: Full-Size Img PowerPoint

Return