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Citation: ZHANG Qiang, LI Rui-Xue, CHEN Xin, HE Xing-Xing, Han Ai-Ling, FANG Guo-Zhen, LIU Ji-Feng, WANG Shuo. Study of Efficiency of Coupling Peptides with Gold Nanoparticles[J]. Chinese Journal of Analytical Chemistry, ;2017, 45(5): 662-667. doi: 10.11895/j.issn.0253-3820.160799 shu

Study of Efficiency of Coupling Peptides with Gold Nanoparticles

Key Laboratory of Food Nutrition and Safety, Ministry of Education of China, Tianjin University of Science and Technology, Tianjin 300457, China

Received Date: 1 November 2016
Revised Date: 16 February 2017

Fund Project: This work was supported by the National Natural Science Foundation of China (No. 21575102).

Fluorescence spectroscopy was used to investigate the efficiency of coupling peptides to gold nanoparticles via 1-(3-dimethylaminopropyl)-3-ethylcarbodiimide hydrochloride-N-hydroxysuccinimide (EDC-NHS). The experiment conditions including buffer solution, pH value and concentrations of buffer solution, concentrations of NHS and EDC, concentration ratios of NHS to EDC, and coupling reaction time on the coupling efficiency were investigated and optimized. The experimental results indicated that the optimized experimental conditions were as follows: 25 mmol/L HEPES buffer solution, pH 7.0; 2∶1 of concentration ratio of NHS to EDC, 0.4 mol/L NHS, 0.2 mol/L EDC, and coupling reaction time of 24 h. This study may provide references for the relative research involving coupling peptide or protein with gold nanoparticles
- 1-(3-Dimethylaminopropyl)-3-ethylcarbodiimide hydrochloride,
- N-Hydroxysuccinimide,
- Gold nanoparticles,
- Peptides
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