Citation: CHANG Yu-Yang, DAI Xiao-Jun, GONG Bo-Lin. Preparation of Carboxybetaine-based Hydrophilic Interaction Chromatographic Column and Its Performance Exploration[J]. Chinese Journal of Analytical Chemistry, ;2017, 45(5): 734-740. doi: 10.11895/j.issn.0253-3820.160787 shu

Preparation of Carboxybetaine-based Hydrophilic Interaction Chromatographic Column and Its Performance Exploration

1.
School of Chemistry&Chemical Engineering, Ningxia University, Yinchuan 750021, China;
2.
School of Chemistry & Chemical Engineering, Beifang University of Nationalities, Yinchuan 750021, China

Received Date: 27 October 2016
Revised Date: 15 March 2017

Fund Project: This work was supported by the National Natural Science Foundation of China (No.1360077) and the Natural Science Foundation of Ningxia (No. ⅣZ13045).

2-(Dimethylamino)ethyl methacrylate and sodium monochloroacetate were employed to synthesize [2-(Methacryloyloxy)ethyl] dimethyl ammonium acetate (CBMA) functional monomer. CBMA was grafted on the surface of silica by surface initiated atom transfer radical polymerization (SI-ATRP) to obtain silica-CBMA hydrophilic interaction stationary phase. Three silica-CBMA stationary phases with different grafted density of CBMA monomer were synthesized in SI-ATRP progress by changing the concentration of CBMA under the same conditions. The ability to separate organic acid compounds of the synthesized silica-CBMA stationary phases was evaluated under different conditions, including effects of pH value, salt concentration and content of water of mobile phase on retention of solutes. The results showed that the stationary phases could effectively separate organic acid compounds in HILIC mode, which followed a mixed mode of chromatography of ion exchange and hydrophilic interaction. The retention of solutes decreased with the increases of salt concentration of mobile phase, which consistent with the characteristics of ion exchange; the pH value of mobile phase had significant influence on ionization of the stationary phase and solutes, i.e., the retention of solutes increased as the increasing of pH value of mobile phase. However, the retention of solutes decreased with the increasing of the content of water in mobile phase, which was the typical characteristic of HILIC. The method of hydrophilic interaction chromatography combined with silica-CBMA stationary phases could conveniently determinate the content of vitamin C and rutin in rutin tablets, providing a new method for the separation and determination of strong polar samples.
- Hydrophilic interaction chromatography,
- Stationary phase,
- Carboxybetaine,
- Atom transfer radical polymerization
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通讯作者: 陈斌, bchen63@163.com

1.
沈阳化工大学材料科学与工程学院沈阳 110142

Preparation of Carboxybetaine-based Hydrophilic Interaction Chromatographic Column and Its Performance Exploration

南开大学师唯/华北电力大学（保定）刘景维：二维配位聚合物中有序的亲锂冠醚位点用于无枝晶锂沉积

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通讯作者: 陈斌, bchen63@163.com