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Citation: CHEN Shi-Fan, YANG Jing-Bo, ZHANG Min, LI Xiao-Ou, LIU Xiao-Feng, GUO Hong-Hua, HE Cheng-Yan, GAO Hong-Wen. Peptidome Analysis of Transudative Pleural Effusion by Ultra-filtration Coupled with Nano-Liquid Chromatography-Tandem Mass Spectrometry[J]. Chinese Journal of Analytical Chemistry, ;2017, 45(2): 224-230. doi: 10.11895/j.issn.0253-3820.160751 shu

Peptidome Analysis of Transudative Pleural Effusion by Ultra-filtration Coupled with Nano-Liquid Chromatography-Tandem Mass Spectrometry

1.
The Second Hospital of Jilin University, Changchun 130021, China;
2.
Tumor Hospital of Jilin Province, Changchun 130021, China;
3.
China-Japan Union Hospital, Jilin University, Changchun 130033, China

Received Date: 11 October 2016
Revised Date: 18 November 2016

Fund Project: This work was supported by the National Natural Science Foundation of China (Nos. 81572082, 81472030) and Jilin Province Science and Technology Department (Nos. 2015101122JC, 20150204001YY, 20140311092YY)

The peptides, proteins and other biological molecules in transudative pleural effusion correlate directly or indirectly with specific physiological and pathological state, reflecting the information regarding the lungs or other parts of the body. In the present study, the peptide fraction in transudative pleural effusion was isolated by ultrafiltration. After desalted and enriched by C₁₈ tips, the peptide mixture was analyzed by nano LC-MS/MS. The results showed that 314 peptides, which were originated from 52 proteins, in pleural transudate were identified. More than half of the peptides were derived from fibrinogen. Many peptides were characterized as displaying ladder sequences. In addition, a large number of proline oxidation modifications were detected in the peptides derived from collagen and fibrinogen. Gene ontology enrichment analysis showed that the most of the proteins extracellular properties of pleural transudate polypeptide components were protein with exocytosis. The study provided a rapid and efficient separation and analysis methods for lung disease markers related peptide compounds in pleural fluid leakage. Also this research provided a rapid and effective method for screening peptide biomarkers related to lung diseases from transudative pleural effusion.
- Peptidome,
- Ultrafiltration,
- Nano-liquid chromatography-tandem mass spectrometry,
- Transudative pleural effusion
1. [1]
2. [2]
3. [3]
4. [4]
5. [5]
6. [6]
7. [7]
8. [8]
9. [9]
10. [10]
11. [11]
12. [12]
13. [13]
14. [14]
15. [15]
16. [16]
17. [17]
18. [18]
19. [19]
20. [20]
21. [21]
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