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Citation: LIU Yu-Jian, LIU Zhi-Min, XU Zhi-Gang. Enantiomeric Separation of Three Kinds of Aromatic Amino Acids by C₁₈ Column Tandem Crown Ether Chiral Column[J]. Chinese Journal of Analytical Chemistry, ;2017, 45(3): 369-373. doi: 10.11895/j.issn.0253-3820.160731 shu

Enantiomeric Separation of Three Kinds of Aromatic Amino Acids by C₁₈ Column Tandem Crown Ether Chiral Column

Faculty of Science, Kunming University of Science and Technology, Kunming 650500, China

Received Date: 28 September 2016
Revised Date: 12 December 2016

Fund Project: This work was supported by the National Natural Science Foundation of China (No. 21565018)

A reversed phase high performance liquid chromatographic (RP-HPLC) method was proposed by using C₁₈ column tandem crown ether chiral column for simultaneous separation of three kinds of aromatic amino acid enantiomers. The chromatographic conditions, including mobile phase proportion, pH, column temperature and flow rate, were optimized. The experimental results showed that the three aromatic amino acid enantiomers could be successfully separated under the optimal conditions such as perchloric acid solution-acetonitrile (86:14, V/V, pH=2) as mobile phase, column temperature of 20℃ and flow rate of 0.4 mL/min. The separation under four HPLC column connection modes was further investigated. The results revealed that different kinds of amino acids could be separated on C₁₈ column, but not for amino acid enantiomers; amino acid enantiomers could be separated on crown ether chiral column, but the chromatographic peaks of each amino acid enantiomers overlapped seriously; baseline separation of three amino acid enantiomers could be obtained under the connection of two columns, but the separation under different column connection sequence almost had no difference except the peak shape.
- Chiral separation,
- Aromatic amino acids,
- Enantiomers,
- Crown ether column
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Enantiomeric Separation of Three Kinds of Aromatic Amino Acids by C18 Column Tandem Crown Ether Chiral Column

南开大学师唯/华北电力大学（保定）刘景维：二维配位聚合物中有序的亲锂冠醚位点用于无枝晶锂沉积

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通讯作者: 陈斌, bchen63@163.com

Enantiomeric Separation of Three Kinds of Aromatic Amino Acids by C₁₈ Column Tandem Crown Ether Chiral Column