Citation: LIU Xiao-Ying, MA Lu-Yao, WANG Li, WANG Xing-Yi, NIE Shao-Ping, XIE Ming-Yong, YIN Jun-Yi. Monosaccharide Composition Analysis of Arabinoxylan by High Performance Anion Exchange Chromatography with Pulsed Amperometric Detection[J]. Chinese Journal of Analytical Chemistry, ;2017, 45(3): 416-422. doi: 10.11895/j.issn.0253-3820.160685 shu

Monosaccharide Composition Analysis of Arabinoxylan by High Performance Anion Exchange Chromatography with Pulsed Amperometric Detection

State Key Laboratory of Food Science and Technology, Nanchang University, Nanchang 330047, China

Received Date: 12 September 2016
Revised Date: 16 December 2016

Fund Project: This work was supported by the National Natural Science Foundation of China (Nos. 31301434, 21564007), the Outstanding Science and Technology Innovation Team Project in Jiangxi Province (No. 20133BCB24001) and the Natural Science Foundation of Jiangxi Province, China (No. 20142BAB214004)

A method of complete acid hydrolysis combined with high performance anion exchange chromatography and pulsed amperometric detection was developed for the monosaccharide composition analysis of arabinoxylan from the seeds of plantago asiatica L. The parameters including hydrolysis methods, acid types, acid concentration, hydrolysis temperature, hydrolysis time and placement time, which would affect the hydrolysis process, were optimized. The results showed that it would have a better hydrolysis effect for polysaccharide from the seeds of Plantago asiatica L. with 2 mol/L H₂SO₄ in an atmospheric oil bath at 120℃ for 2 hours. However, the placement time for diluted solution of the hydrolyzed polysaccharide should be less than 6 hours. The polysaccharide was mainly composed of Arabinose (8.89%) and Xylose (41.52%) and Galacturonic acid (0.73%). Glcuronic acid (3.44%) was detected simultaneously, and there were also trace amounts of Galatose and Glucose. The results were reproducible. Other arabinoxylans from Panicummiliaceum L. shell, Avena sativa L. bran and Hordeum vulgare L. were taken for monosaccharide compositions analysis under the optimal hydrolysis conditions and the analysis results were good. This study would provide a good reference for monosaccharides composition analysis of arabinoxylans from various sources.
- Arabinoxylan,
- Seeds from plantago asiatica L.,
- High performance anion exchange chromatography,
- Hydrolysis condition,
- Monosaccharide composition
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