Citation: LIU Yu-Fei, XUE Jin-Tao, YAN Hui-Juan, YANG Li-Juan, LIU Wei, SUN Xiang-De. A Dual-signal Amplification Method for DNA Detection Based on Exonuclease Ⅲ and Fluorescence Quenching Ability of MoS₂ Nanosheet[J]. Chinese Journal of Analytical Chemistry, ;2017, 45(3): 303-308. doi: 10.11895/j.issn.0253-3820.160642 shu

A Dual-signal Amplification Method for DNA Detection Based on Exonuclease Ⅲ and Fluorescence Quenching Ability of MoS₂ Nanosheet

School of Pharmacy, Xinxiang Medical University, Xinxiang 453003, China

Received Date: 29 August 2016
Revised Date: 16 December 2016

Fund Project: This work was supported by the National Natural Science Foundation of China (No. 21505114) and the Key Project of Henan Provincial Department of Education (No. 14A150010)

A highly sensitive and selective DNA biosensor is described based on the fluorescence quenching ability of MoS₂ nanosheet and exonuclease Ⅲ (Exo Ⅲ) assisted dual-signal amplification. In this sensor, the fluorescence probes (HP1 and HP2) cannot be degraded by Exo Ⅲ due to the 3'-termini protrusion and thus are adsorbed on the surface of MoS₂ nanosheets, which will result in the quenching of MoS₂ nanosheets toward the probes and induce a low fluorescent signal. The presence of the target DNA leads to the desorption of probes on the surface of MoS₂ nanosheets due to the hybridization toward probes, generating many fluorescent fragments by Exo Ⅲ digestion and inducing dual-signal amplification. This method can improve the sensitivity and detection limit compared with single amplification method, and shows excellent selectivity in the discrimination of single base mismatched targets. On the basis of the significantly high sensitivity, the developed biosensor can be potentially extended to detect various DNA targets with excellent sequence selectivity.
- DNA sensor,
- Molybdenum disulfide nanosheet,
- Exonuclease Ⅲ,
- Dual-signal amplification method,
- Quenching ability
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A Dual-signal Amplification Method for DNA Detection Based on Exonuclease Ⅲ and Fluorescence Quenching Ability of MoS2 Nanosheet

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通讯作者: 陈斌, bchen63@163.com

A Dual-signal Amplification Method for DNA Detection Based on Exonuclease Ⅲ and Fluorescence Quenching Ability of MoS₂ Nanosheet