Citation: GU Bei-Qiao, MEI Guang-Ming, ZHANG Xiao-Jun, HE Yi-Na, YAN Zhong-Yong, ZHU Jing-Ping. Determination of 8 Kinds of Cephalosporins in Aquatic Products by Multi-walled Carbon Nanotubes Solid Phase Extraction-Ultra High Performance Liquid Chromatography-Mass Spectrometry[J]. Chinese Journal of Analytical Chemistry, ;2017, 45(3): 381-388. doi: 10.11895/j.issn.0253-3820.160633 shu

Determination of 8 Kinds of Cephalosporins in Aquatic Products by Multi-walled Carbon Nanotubes Solid Phase Extraction-Ultra High Performance Liquid Chromatography-Mass Spectrometry

Marine Fisheries Research Institute of Zhejiang, Key Lab of Mariculture and Enhancement of Zhejiang Province, Zhoushan 316021, China

Received Date: 23 August 2016
Revised Date: 23 November 2016

Fund Project: This work was supported by the Science & Technology Department Project of Zhejiang Province, China (Nos. 2016C32074, 2016F30022)

An ultra-high performance liquid chromatography-mass spectrometry (UPLC-MS/MS) method was developed for the determination of 8 kinds of cephalosporins, cefoperazone, cefquinome, cefalonium, cefazolin, cefapirin, Ceftiofur, cefpirome and cefalexin, in edible parts of aquatic products. The samples were extracted with acetonitrile-water and cleaned up by multi-walled carbon nanotubes (MWCNTs) SPE cartridge. All the target compounds were separated on an Acquity Xselect CSH C₁₈ column with gradient elution by using acetonitrile and 0.1% formic acid aqueous as eluent, and detected by UPLC-MS/MS under ESI⁺ ionization and MRM mode. Under optimized conditions, this method had a good linearity (R²≥0.995) and the limits of quantification were in the range of 2-10 μg/kg (S/N=10). The recoveries of the method for the target compounds spiked at three different levels were 67.3%-94.2% with the relative standard deviations (RSDs) of 3.3%-14%. The method had the characteristics of low cost, high accuracy and good precision, and could meet the requirements of cephalosporins determination.
- Multi-walled carbon nanotubes,
- Ultra-high performance liquid chromatography-mass spectrometry,
- Aquatic product,
- Cephalosporins
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