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Citation: SHAN Xue-Feng, ZHANG Shui-Mei, CAI Zhi-Hui, LING Qin-Jie, HUANG Zhi. Infrared Spectrum, UV-visible Absorption Spectral Characteristics and Proteomic Analysis of Selenium Enriched Spirulina Platensis[J]. Chinese Journal of Analytical Chemistry, ;2016, 44(12): 1814-1819. doi: 10.11895/j.issn.0253-3820.160496 shu

Infrared Spectrum, UV-visible Absorption Spectral Characteristics and Proteomic Analysis of Selenium Enriched Spirulina Platensis

School of Life Science and Technology, Jinan University, Guangzhou 510632, China

Received Date: 29 June 2016
Revised Date: 17 October 2016

Fund Project: This work was supported by the National Natural Science Foundation of China (No.81570397), the key project of Natural Science Foundation of Guandong Provine (No. 2014A030311026), the key Project of Marine fishery Science and Technology of Guangdong Province (No. A201501C07) and the Fundamental Research Funds for the Central Universities of China (Jinan University, No. 21611611).

Infrared spectrum, UV-visible absorption spectrum and differential expression of proteome between Spirulina platensis (SP) and selenium enriched SP (Se-SP) were investigated. The selenium containing proteins were separated from the total proteins in Se-SP by a proteomic approach of two-dimensional electrophoresis (2DE), identified by matrix assisted laser desorption ionization time-of-flight mass spectrometry (MALDI-TOF-MS), and determined by inductively coupled plasma mass spectrometry (ICP-MS). Results showed that Se enrichment cultivation could significantly up-regulate the expression of pigment proteins such as α/β subunits of phycocyanin (PC) and allophycocyanin (APC) which involved in light harvest. The large subunits of Rbisco and superoxide dismutase were also increased obviously in Se-SP. In addition, it was found that Se was abundantly distributed in the range of pH 4-7 and M_W of 14.4-43 kDa in the 2DE gel. Individuals of Se containing protein should be explored in Se-SP in further studies.
- Selenium enriched Spirulina platensis,
- Spectral Analysis,
- Two dimensional electrophoresis,
- Selenium containing proteins,
- Proteomics
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