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Citation: HE Zhong-Mei, LYU Na, NAN Min-Lun, ZHAO Yu-Wei, HE Yu-Fang, MENG Ling-Wen, SUN Jia-Ming, ZHANG Lian-Xue. Screening and Structure Characterization of Acetylcholinesterase Inhibitors from Total Alkaloids of Fibraurea recisa Pierre. by Target Molecule Affinity-Liquid Chromatography-Tandem Mass Spectrometry[J]. Chinese Journal of Analytical Chemistry, ;2017, 45(2): 211-216. doi: 10.11895/j.issn.0253-3820.160460 shu

Screening and Structure Characterization of Acetylcholinesterase Inhibitors from Total Alkaloids of Fibraurea recisa Pierre. by Target Molecule Affinity-Liquid Chromatography-Tandem Mass Spectrometry

1.
College of Chinese Medicine Materials, Jilin Agricultural University, Changchun 130118, China;
2.
Academy of Chinese Medical Sciences of Jilin Province, Changchun 130012, China;
3.
Changchun University of Chinese Medicine, Changchun 130117, China

Received Date: 14 June 2016
Revised Date: 7 November 2016

A target molecule affinity-ultrafiltration liquid chromatography-electrospray ionization tandem mass spectrometric (LC-ESI-MSⁿ) method was established for rapid screening acetylcholinesterase (AChE) inhibitors from total alkaloids of fibraurea recisa Pierre. A total of 12 potential inhibitors were screened from Fibraurea recisa Pierre. and 6 compounds were identified including palmatine, berberine, jatrorrhizine, palmatrubine, 7,8-dihydro-8-hydroxyberberine and groenlandicine. The AChE inhibitory activity of these 6 compounds was validated in vitro. Palmatine showed the strongest inhibitory activity for AChE, which was stronger than that of donepezil hydrochloride, demonstrating the potential of palmatine as anti-Alzheimer's drug. This method is simple, rapid, and accurate for directly screening active ingredients which can inhibit AChE from complex extract of traditional Chinese medicines.
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