Citation: WU Cai-Ye, MIAO Yang-Bao, ZHU Yun-Yun, GAN Ning, OU Chang-Rong, CAO Jin-Xuan. A Colorimetric Bionic Aptasensor Base on Magnetic Metal Organic Framework Compound-Aptamer Probe for Specific Detection of Chloramphenicol[J]. Chinese Journal of Analytical Chemistry, ;2016, 44(12): 1820-1827. doi: 10.11895/j.issn.0253-3820.160449 shu

A Colorimetric Bionic Aptasensor Base on Magnetic Metal Organic Framework Compound-Aptamer Probe for Specific Detection of Chloramphenicol

1.
School of Marine Sciences, Ningbo University, Ningbo 315211, China;
2.
The School of Materials Science and Chemical Engineering, Ningbo University, Ningbo 315211, China

Received Date: 12 June 2016
Revised Date: 15 August 2016

Fund Project: This work was supported by the National Natural Science Foundation of China (Nos. 31271890, 31501519).

A colorimetric bionic aptasensor based on magnetic metal organic framework (MOF) compound-aptamer bionic probe was designed for detecting chloramphenicol (CAP). In this assay, the aptamer was labeled on the magnetic beads as capture probe and its complementary strand (cDNA) was immobilized on MOF as the nanotracer composite probe, and the ferromagnetic biomimetic composite probe was obtained by hybridizing the capture probe with the tracer. When the composite probe solution was incubated with CAP, the aptamer on the capture probes preferentially combines with CAP, and the signal tag was substituted into supernatant after magnetic separation. The supernatant containing numerous MOF could efficiently catalyze tetramethylbenzidine (TMB)-H₂O₂ solution for the color development quantified by ultraviolet-visible spectroscopy. Under the optimal reaction conditions, the experimental results showed the CAP detection owned a linear range of 0.001-10 ng/mL and limit of detection was 0.3 pg/mL (S/N=3). The recoveries of standard addition in the actual samples were 86.9%-93.5% with no interference of other antibiotics. Besides, the results of this method for CAP detection in the milk samples agreed well with the ELISA results. Compared with the enzyme labeled probe, the non-enzyme labeled bionic probe using the magnetic separation greatly simplifies the pre-processing step, and it has many advantages such as high catalytic activity and low cost, and can be applied to the field detection of CAP in food with rapidity, high specificity and high sensitivity.
- Magnetic metal organic framework compound,
- Magnetic capture probe,
- Chloramphenicol,
- Aptamer
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沈阳化工大学材料科学与工程学院沈阳 110142