Citation: DENG Li-Hua, DAI Jin-Bo, XU Zhen-Lin, YANG Jin-Yi, WANG Hong, XIAO Zhi-Li, LEI Hong-Tao, SUN Yuan-Ming, SHEN Yu-Dong. Application of Time-resolved Fluroimmunoassay for Determination of Furaltadone Metabolite 3-Amino-5-morpholinomethyl-2-oxazolidinone[J]. Chinese Journal of Analytical Chemistry, ;2016, 44(8): 1286-1290. doi: 10.11895/j.issn.0253-3820.160192 shu

Application of Time-resolved Fluroimmunoassay for Determination of Furaltadone Metabolite 3-Amino-5-morpholinomethyl-2-oxazolidinone

College of Food Science, South China Agricultural University, Guangdong Provincial Key Laboratory of Food Quality and Safety, Guangzhou, 510642, China

Received Date: 15 March 2016
Revised Date: 13 April 2016

Figures(5)

To detect furaltadone metabolite 3-amino-5-morpholinomethyl-2-oxazolidinone (AMOZ) in fish sample, an Eu³⁺ labeling time-resolved fluoroimmunoassay (TRFIA) was developed. The effects of experimental conditions including AMOZA-OVA concentration, dilution of antibody, and reaction time on the sensitivity of TRFIA were explored. The results showed that the optimized assay conditions were as follows:the AMOZA-OVA concentration was 0.25 μg/mL; the antibody was diluted 5×10⁴ folds, and the competitive reaction time was 50 min. Under optimal conditions, the method showed a detection limit of 0.01 ng/mL, an IC₅₀ of 0.26 ng/mL and a linear range (IC₂₀-IC₈₀) of 0.025-2.83 ng/mL. The recoveries of AMOZ in fish at three spiked levels ranged from 78.0% to 86.0%, and the relative standard deviations were less than 15%. Good correlation between the ic-TRFIA and high performance liquid chromatography-tandem mass spectrometry was obtained for spiked food samples. The proposed ic-TRFIA method was suited for the determination of AMOZ residue in food samples.
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