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Citation: JIANG Zhen-Zuo, ZHANG Chun-Ze, WANG Yue-Fei, FU Wen-Zheng. Establishment of a Novel Diagnostic Method for Colorectal Cancer Based on Metabolites of Intestinal Microorganism[J]. Chinese Journal of Analytical Chemistry, ;2016, 44(8): 1178-1184. doi: 10.11895/j.issn.0253-3820.160148 shu

Establishment of a Novel Diagnostic Method for Colorectal Cancer Based on Metabolites of Intestinal Microorganism

1.
Tianjin State Key Laboratory of Modern Chinese Medicine, Tianjin University of Traditional Chinese Medicine, Tianjin 300193, China;
2.
Research and Development Center of Traditional Chinese Medicine, Tianjin International Joint Academy of Biotechnology and Medicine, Tianjin 300457, China;
3.
Department of Anorectal Surgery, Tianjin Union Medicine Centre, Tianjin 300121, China

Received Date: 2 March 2016
Revised Date: 11 April 2016

Fund Project: This work was supported by the National Science and Technology Major Projects for "Major New Drugs Innovation and Development" (No.2015ZX09J15102-004-004) and the Science and Technology Fund of Key Project for Tianjin Health and Family Planning Commission (No.2010KG206)

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A rapid gas chromatographic (GC) method was established for the determination of short-chain fatty acids (SCFAs) in human feces. Feces samples were directly extracted by 1% HCl-75% ethanol solution, and then centrifuged at high speed for GC analysis. The chromatographic separation was performed on a DB-FFAP capillary column (30 m×0.25 mm×0.25 μm) with a temperature program (initial temperature at 50℃ held for 1 min, ramped to 190℃ at 10℃/min). The injection port temperature was 250℃ with split ratio at 50:1. The carrier gas was high purity nitrogen with a constant linear velocity at 1.0 mL/min. A flame ionization detector was employed to quantify SCFAs. The proposed method had been certified by systematic method validation, and used to determine feces samples. Subsequently, the health volunteer and colorectal cancer patient groups could be distinguished successfully by multivariate statistical analysis. Compared with health volunteers, the acetic acid and butyric acid of feces from colorectal cancer patients were reduced obviously, indicating that SCFAs particularly butyric acid could be considered as candidate markers for colorectal cancer diagnosis. In summary, this study provides a rapid method for the determination of SCFAs in feces form health volunteers and colorectal cancer patients. The method had a prospect for screening and diagnosing colorectal cancer rapidly.
- Gas chromatography,
- Gut microbiota,
- Short-chain fatty acids,
- Colorectal cancer,
- Diagnosis method
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