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Citation: ZHAO Zhi-Jie, ZHANG Ping, YANG Jia-Xiang, HAO Chang-Chun, ZHOU Xing-Fei, LI Bin. High-Resolution Imaging of Single-Molecule Immunoglobulin G Antibodies with Atomic Force Microscopy in Liquid[J]. Chinese Journal of Analytical Chemistry, ;2016, 44(8): 1215-1220. doi: 10.11895/j.issn.0253-3820.160143 shu

High-Resolution Imaging of Single-Molecule Immunoglobulin G Antibodies with Atomic Force Microscopy in Liquid

1.
Ningbo University's Faculty of Science, Ningbo University, Ningbo 315211, China;
2.
School of Life Science, Inner Mongolia Agricultural University, Hohhot 010018, China;
3.
School of Physics and Information Technology, Shaanxi Normal University, Xi'an 710062, China;
4.
Division of Physical Biology & Bioimaging Center, Shanghai Synchrotron Radiation Facility, Chinese Academy of Sciences, Key Laboratory of Interfacial Physics and Technology, Shanghai Institute of Applied Physics, Chinese Academy of Sciences, Shanghai 201800, China

Received Date: 9 February 2016
Revised Date: 12 May 2016

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Atomic force microscopy (AFM) is one of important tools for studying the structure and function of biomolecules due to its nanoscale capability. A well prepared sample was the primary key issue for high-resolution imaging macromolecules. Here, by using self-assembly technology of DNA origami, antigens were modified on DNA origami. Afterward, antibodies reacted specifically with antigens by a molecular recognition way. Then, a nanostructure formed from DNA origami and antigen-antibody complexes. By the aid of adsorption character of DNA origami, individual antibodies could selectively adsorb on mica surface. As a result, the ultrastructural morphology image of a single IgG molecule, the characteristic "Y-shaped" domains, was resolved in liquid. The way for adsorbing biomolecules on the mica surface in a liquid environment was simple and convenient, and it may be useful to detect and measure biomoleculers at the singe-molecule level with AFM.
- Atomic force microscopy,
- Single-molecule,
- High-resolution imaging,
- Immunoglobulin G,
- DNA origami
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