Citation: ZHU Hua-Dong, CHEN Yong-Lei, CHEN Hong-Li, CHEN Xing-Guo. On-line Study of Aesculin and Aesculetin Hydrolysis Using Sweeping-Flow Injection-chip Micellar Electrokinetic Chromatography[J]. Chinese Journal of Analytical Chemistry, ;2016, 44(4): 597-603. doi: 10.11895/j.issn.0253-3820.160142 shu

On-line Study of Aesculin and Aesculetin Hydrolysis Using Sweeping-Flow Injection-chip Micellar Electrokinetic Chromatography

1.
College of Chemistry and Chemical Engineering, Lanzhou University, Lanzhou 730000, China

Corresponding author: CHEN Xing-Guo,
Received Date: 28 February 2016
Available Online: 6 March 2016
Fund Project: 本文系国家自然科学基金(No.21375053)资助 (No.21375053)

Aesculin and aesculetin hydrolysis were studied on-line using sweeping-flow injection-chip micellar electrokinetic chromatography(Sweeping-FI-chip MEKC). With this method, all the analytes in the sample are well separated within 5 min, and a sampling rate of 12 per hour is achieved. At this sampling frequency, a rate constant can be obtained within 30 min. In contrast to off-line methods, it was quite time-saving. And the aesculin hydrolysis mixture solution is directly injected and analyzed without quenching the reaction, thus the information of the hydrolysis process can be obtained from the electrophorogram of a sequence injection. Under the optimum conditions, the hydrolysis rate constants for aesculin at 25, 30, 35, 40 and 45℃ using 0.1 mol/L KOH as hydrolyte are obtained as 3.65×10^-2/min, 5.24×10^-2/min, 7.12×10^-2/min, 10.5×10^-2/min and 16.3×10^-2/min, respectively. The activation energy for aesculin hydrolysis is 58.57 kJ/mol. The hydrolysis rate constants for aesculetin at 15, 20, 25, 30 and 35℃ using 10 mmol/L KOH as hydrolyte are obtained as 2.26×10^-2/min, 2.85×10^-2/min, 3.55×10^-2/min, 4.38×10^-2/min and 5.29×10^-2/min, respectively. The activation energy for aesculin hydrolysis is 31.55 kJ/mol.
- Sweeping-flow injection-chip micellar electrokinetic chromatography,
- On-line hydrolysis,
- Aesculin,
- Aesculetin,
- Rate constant
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