Citation: CHEN Xi, CHEN Jin, ZHANG Hui-Yan, WANG Fu-Bing, WANG Fang-Fang, JI Xing-Hu, HE Zhi-Ke. Colorimetic Detection of Alkaline Phosphatase on Microfluidic Paper-based Analysis Devices[J]. Chinese Journal of Analytical Chemistry, ;2016, 44(4): 591-596. doi: 10.11895/j.issn.0253-3820.160140 shu

Colorimetic Detection of Alkaline Phosphatase on Microfluidic Paper-based Analysis Devices

1.
¹ Key Laboratory of Analytical Chemistry for Biology and Medicine, Ministry of Education, College of Chemistry and Molecular Sciences, Wuhan University, Wuhan 430072, China;
2.
² Department of Laboratory Medicine, Zhongnan Hospital of Wuhan University, Wuhan 430071, China

Corresponding author: JI Xing-Hu,
Received Date: 25 February 2016
Available Online: 7 March 2016
Fund Project: 本文系国家自然科学基金(No.21205089) (No.21205089)教育部高等学校博士学科点专项科研基金(No.20120141120036)资助项目 (No.20120141120036)

A low-cost, simple and sensitive colorimetric detection method of alkaline phosphatase(ALP) has been developed on microfluidic paper-based analysis devices. In a typical colorimetric detection, 5-bromo-4-chloro-3-indolyl phosphate disodium salt(BCIP) and nitro blue tetrazolium(NBT) were firstly added onto the circle array zones of paper devices. Then ALP solutions were spotted to the array zones to perform the colorimetric reaction. The color results were recorded by both Gel Documentation systems and a common camera, and analyzed with Quantity One software. All the reaction conditions were aptimized. Under the optimal conditions, the colorimetric intensity showed a linear correlation to the concentration of ALP in the range of 1.5 to 20 U/L with the limit of detection(LOD) of 0.78 U/L(3σ), which was about two orders of magnitude lower than that of the reported method on μPADs. Besides, it could be applied in spiked real sample analysis with a satisfactory result. In addition, a dual-color array-based paper strip has been fabricated for the semiquantitative detection of ALP. The approximate activity of ALP could be simply distinguished by observing the color change with naked eyes.
- Microfluidic paper-based analysis devices,
- Colorimetric detection,
- Alkaline phosphatase
1. [1]
  1 Fernandez N J, Kidney B A. Vet. Clin. Pathol., 2007, 36:223-233
2. [2]
  2 Jia L, Xu J P, Li D, Pang S P, Fang Y, Song Z G, Ji J. Chem. Commun., 2010, 46:7166-7168
3. [3]
  3 Ooi K, Shiraki K, Morishita Y, Nobori T. J. Clin. Lab. Anal., 2007, 21:133-139
4. [4]
  4 Hou F, Zhang Q, Yang J, Li X, Yang X, Wang S, Cheng Z. Biomed. Microdevices, 2012, 14:729-737
5. [5]
  5 Wei H, Chen C, Han B, Wang E. Anal. Chem., 2008, 80:7051-7055
6. [6]
  6 Gyurcsanyi R E, Bereczki A, Nagy G, Neuman M R, Lindner E. Analyst, 2002, 127:235-240
7. [7]
  7 Kim T, Kim H, Choi Y, Kim Y. Chem. Commun., 2011, 47:9825-9827
8. [8]
  8 Hallaway B J, O'Kane D J. Method. Enzymol., 2000, 305:391-401
9. [9]
  9 Ruan C, Wang W, Gu B. Anal. Chem., 2006, 78:3379-3384
10. [10]
  10 Li X, Tian J F, Shen W. Cellulose, 2010, 17:649-659
11. [11]
  11 Martinez A W, Phillips S T, Whitesides G M, Carrilho E. Anal. Chem., 2010, 82:3-10
12. [12]
  12 He Y, Wu Y, Fu J Z, Wu W B. RSC Adv., 2015, 5, 78109-78127
13. [13]
  13 Xia Y Y, Si J, Li Z Y. Biosens. Bioelectron., 2016, 77:774-789
14. [14]
  14 Carrilho E, Martinez A W, Whitesides G M. Anal. Chem., 2009, 81:7091-7095
15. [15]
  15 Lu Y, Shi W W, Qin J H, Lin B C. Anal. Chem., 2010, 82:329-335
16. [16]
  16 Wang S M, Ge L, Song X R, Yan M, Ge S G, Yu J H, Zeng F. Analyst, 2012, 137:3821-3827
17. [17]
  17 Kao P K, Hsu C C. Anal. Chem., 2014, 86:8757-8762
18. [18]
  18 Jiang Y, Hao Z X, He Q H, Chen H W. RSC Adv., 2016, 6:2888-2894
19. [19]
  19 Li X, Tian J F, Nguyen T, Shen W. Anal. Chem., 2008, 80:9131-9134
20. [20]
  20 Cheng C M, Martinez A W, Gong J L, Mace C R, Phillips S T, Carrilho E, Mirica K A, Whitesides G M. Angew. Chem. Int. Ed., 2010, 49:4771-4774
21. [21]
  21 Vella S J, Beattie P, Cademartiri R, Laromaine A, Martinez A W, Phillips S T, Mirica K A, Whitesides G M. Anal. Chem., 2012, 84:2883-2891
22. [22]
  22 Chen X, Chen J, Wang F, Xiang X, Luo M, Ji X, He Z. Biosens. Bioelectron., 2012, 35:363-368
23. [23]
  23 ZHANG Hui-Yan, ZHANG Zhen, JI Xing-Hu, HE Zhi-Ke. Chinese J. Anal. Chem., 2014, 42(9):1276-1280 张慧妍, 张珍, 吉邢虎, 何治柯. 分析化学, 2014, 42(9):1276-1280
24. [24]
  24 Fanjul-Bolado P, Hernandez-Santos D, Gonzalez-Garcia M B, Costa-Garcia A. Anal. Chem., 2007, 79:5272-5277
1. [1]
  Hong LI , Xiaoying DING , Cihang LIU , Jinghan ZHANG , Yanying RAO . Detection of iron and copper ions based on gold nanorod etching colorimetry. Chinese Journal of Inorganic Chemistry, 2024, 40(5): 953-962. doi: 10.11862/CJIC.20230370
2. [2]
  Qin Tu , Anju Tao , Tongtong Ma , Jinyi Wang . Innovative Experimental Teaching of Escherichia coli Detection Based on Paper Chip. University Chemistry, 2024, 39(6): 271-277. doi: 10.3866/PKU.DXHX202309062
3. [3]
  Liwei Wang , Guangran Ma , Li Wang , Fugang Xu . A Comprehensive Analytical Chemistry Experiment: Colorimetric Detection of Vitamin C Using Nanozyme and Smartphone. University Chemistry, 2024, 39(8): 255-262. doi: 10.3866/PKU.DXHX202312094
4. [4]
  Min Gu , Huiwen Xiong , Liling Liu , Jilie Kong , Xueen Fang . Rapid Quantitative Detection of Procalcitonin by Microfluidics: An Instrumental Analytical Chemistry Experiment. University Chemistry, 2024, 39(4): 87-93. doi: 10.3866/PKU.DXHX202310120
5. [5]
  Zhibei Qu , Changxin Wang , Lei Li , Jiaze Li , Jun Zhang . Organoid-on-a-Chip for Drug Screening and the Inherent Biochemistry Principles. University Chemistry, 2024, 39(7): 278-286. doi: 10.3866/PKU.DXHX202311039
6. [6]
  Endong YANG , Haoze TIAN , Ke ZHANG , Yongbing LOU . Efficient oxygen evolution reaction of CuCo₂O₄/NiFe-layered bimetallic hydroxide core-shell nanoflower sphere arrays. Chinese Journal of Inorganic Chemistry, 2024, 40(5): 930-940. doi: 10.11862/CJIC.20230369
7. [7]
  Liang MA , Honghua ZHANG , Weilu ZHENG , Aoqi YOU , Zhiyong OUYANG , Junjiang CAO . Construction of highly ordered ZIF-8/Au nanocomposite structure arrays and application of surface-enhanced Raman spectroscopy. Chinese Journal of Inorganic Chemistry, 2024, 40(9): 1743-1754. doi: 10.11862/CJIC.20240075
8. [8]
  Quanliang Chen , Zhaohui Zhou . Research on the Active Site of Nitrogenase over Fifty Years. University Chemistry, 2024, 39(7): 287-293. doi: 10.3866/PKU.DXHX202310133
9. [9]
  Heng Zhang . Determination of All Rate Constants in the Enzyme Catalyzed Reactions Based on Michaelis-Menten Mechanism. University Chemistry, 2024, 39(4): 395-400. doi: 10.3866/PKU.DXHX202310047
10. [10]
  Yan LIU , Jiaxin GUO , Song YANG , Shixian XU , Yanyan YANG , Zhongliang YU , Xiaogang HAO . Exclusionary recovery of phosphate anions with low concentration from wastewater using a CoNi-layered double hydroxide/graphene electronically controlled separation film. Chinese Journal of Inorganic Chemistry, 2024, 40(9): 1775-1783. doi: 10.11862/CJIC.20240043
11. [11]
  Zhao Lu , Hu Lv , Qinzhuang Liu , Zhongliao Wang . Modulating NH₂ Lewis Basicity in CTF-NH₂ through Donor-Acceptor Groups for Optimizing Photocatalytic Water Splitting. Acta Physico-Chimica Sinica, 2024, 40(12): 2405005-. doi: 10.3866/PKU.WHXB202405005
12. [12]
  Renxiao Liang , Zhe Zhong , Zhangling Jin , Lijuan Shi , Yixia Jia . A Palladium/Chiral Phosphoric Acid Relay Catalysis for the One-Pot Three-Step Synthesis of Chiral Tetrahydroquinoline. University Chemistry, 2024, 39(5): 209-217. doi: 10.3866/PKU.DXHX202311024
13. [13]
  Renqing Lü , Shutao Wang , Fang Wang , Guoping Shen . Computational Chemistry Aided Organic Chemistry Teaching: A Case of Comparison of Basicity and Stability of Diazine Isomers. University Chemistry, 2025, 40(3): 76-82. doi: 10.12461/PKU.DXHX202404119
14. [14]
  Chunmei GUO , Weihan YIN , Jingyi SHI , Jianhang ZHAO , Ying CHEN , Quli FAN . Facile construction and peroxidase-like activity of single-atom platinum nanozyme. Chinese Journal of Inorganic Chemistry, 2024, 40(9): 1633-1639. doi: 10.11862/CJIC.20240162
15. [15]
  Zhuoya WANG , Le HE , Zhiquan LIN , Yingxi WANG , Ling LI . Multifunctional nanozyme Prussian blue modified copper peroxide: Synthesis and photothermal enhanced catalytic therapy of self-provided hydrogen peroxide. Chinese Journal of Inorganic Chemistry, 2024, 40(12): 2445-2454. doi: 10.11862/CJIC.20240194

Get Citation

PDF

XML

Metrics

PDF Downloads(1)
Abstract views(383)
HTML views(53)

通讯作者: 陈斌, bchen63@163.com

1.
沈阳化工大学材料科学与工程学院沈阳 110142