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Citation: SHAN Bing-Hui, WANG Chao-Zhan, WEI Yin-Mao. Preparation and Adsorption Property of bis (5-Methyltetrazolium) amine-bonded Metal Ion Affinity Magnetic Nanoparticles for Protein[J]. Chinese Journal of Analytical Chemistry, ;2016, 44(7): 1119-1124. doi: 10.11895/j.issn.0253-3820.160125 shu

Preparation and Adsorption Property of bis (5-Methyltetrazolium) amine-bonded Metal Ion Affinity Magnetic Nanoparticles for Protein

College of Chemistry and Materials Science, Northwest University, Xi'an 710127, China

Received Date: 25 February 2016
Revised Date: 22 April 2016

Fund Project: This work was supported by the National Natural Science Foundation of China (Nos. 21275115, 21475104, 21575114)

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A bis(5-methyltetrazolium)amine-bonded magnetic nanoparticle adsorbent was prepared by chemically modifying magnetic nanoparticles Fe₃O₄@SiO₂ via four steps chemical reactions. The physical properties of the adsorbent were characterized by X-ray photoelectron spectroscopy (XPS) and Zeta potential. The static adsorption behavior of lysozyme, cytochrome C and chymotrypsin on the chelated Cu(Ⅱ) adsorbent, as well as the influences of the pH value of solution, ion strength and initial protein concentration on the adsorption capacity were evaluated with batch method. The results illustrated that the adsorption of protein proceeded via metal coordination mechanism and was also in accordance with Langmuir adsorption model, and the maximum adsorption capacities of lysozyme, cytochrome C and chymotrypsin were calculated to be 20.0 mg/g, 13.5 mg/g, and 17.9 mg/g, respectively. In addition, the chelated Cu(Ⅱ) adsorbent was employed to adsorb protein mixture, showing that this new type of adsorbent had selective adsorption to protein mixture. These results illustrated that the metal-chelated adsorbent had potential application value in selectively separating and enriching proteins.
- Magnetic separation,
- Immobilized metal ion affinity,
- Adsorbent,
- Protein
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