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Citation: CUI Yin-Yin, CHEN Zeng-Ping, YAN Xiu-Fang, YU Ru-Qin. Quantification of Nitrite Ion in Environmental Water Samples by Ratiometric Fluorescence Indicator in Combination with Quantitative Fluorescence Model[J]. Chinese Journal of Analytical Chemistry, ;2016, 44(8): 1250-1256. doi: 10.11895/j.issn.0253-3820.160063 shu

Quantification of Nitrite Ion in Environmental Water Samples by Ratiometric Fluorescence Indicator in Combination with Quantitative Fluorescence Model

State Key Laboratory of Chemo/Biosensing and Chemometrics, College of Chemistry and Chemical Engineering, Hunan University, Changsha 410082, China

Received Date: 24 January 2016
Revised Date: 9 March 2016

Fund Project: This work was supported by the National Natural Science Foundation of China (Nos. 21275046, 21475039), the National Major Scientific Instruments and Equipments Special Project (No. 2011YQ0301240102), the Natural Science Foundation of Hunan Province, China (No. 2015JJ2028), the Ph.D. Programs Foundation of Ministry of Education of China (No. 20130161110027) and the Program for New Century Excellent Talents in University (No. NCET-12-0161)

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A novel method was proposed for the quantification of nitrite ion in aqueous samples by the combination of an advanced quantitative fluorescence model with ratiometric fluorescence indicator 2,3-diaminonaphthalene. The proposed method was tentatively applied to direct quantitative determination of nitrite ion in turbid environmental water samples containing both scatterers and absorbers; and its performance was thoroughly investigated and evaluated. Experimental results showed that the proposed method could realize accurate quantitative determination of nitrite ion in environmental water samples with recovery rates in the range of 90.8%-103.0%, which were comparable to the corresponding values of HPLC-DAD experiments. The limit of detection and limit of quantification of the proposed method were estimated to be 1.9 and 5.8 nmo/L, respectively.
- Ratiomatric fluorescence indicator,
- Nitrite ion,
- Quantitative analysis,
- Quantitative fluorescence model
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