Citation: MENG Fan-Da, HUO Wei-Song, HE Mei-Lin, LI Hao, LIAN Jie, SHI Xi-Zeng, GAO Yun-Hua. A Microfluidic Fluorescence Immunoassay Test Card for Rapid Detection of Heart-type Fatty Acid Binding Protein[J]. Chinese Journal of Analytical Chemistry, ;2016, 44(4): 633-639. doi: 10.11895/j.issn.0253-3820.160045 shu

A Microfluidic Fluorescence Immunoassay Test Card for Rapid Detection of Heart-type Fatty Acid Binding Protein

MENG Fan-Da ^1,2 ,
HUO Wei-Song ¹ ,
HE Mei-Lin ^1,2 ,
LI Hao ^1,2 ,
LIAN Jie ³ ,
SHI Xi-Zeng ⁴ ,
GAO Yun-Hua ^1,,

1.
¹ Key Laboratory of Photochemical Conversion and Optoelectronic Materials, Technical Institute of Physics and Chemistry, Chinese Academy of Sciences, Beijing 100190, China;
2.
² University of Chinese Academy of Sciences, Beijing 100049, China;
3.
³ Department of Forensic Science, People's Public Security University of China, Beijing 100038, China;
4.
⁴ Dongguan Bosh Biotechnologies, LTD, Dongguan 523808, China

Corresponding author: GAO Yun-Hua,
Received Date: 16 January 2016
Available Online: 3 March 2016
Fund Project: 本文系东莞市重大科技项目(No.2014215102) (No.2014215102)SEM技术创新基金(No.14C26214402622)资助 (No.14C26214402622)

A kind of easily assembled self-driving test card was developed for the rapid detection and quantification of heart-type fatty acid binding protein(H-FABP) by using time-resolved fluorescence microspheres as signal probes. It was easy to make microfluidic channel structures with double-sided adhesive and cut out of test card substrate and cover based on polymethyl methacrylate(PMMA) material by laser cutting technology. The membrane of polystyrene containing maleic anhydride functional groups was coated on PMMA plate surface by dip-coating method. Further, the capture antibody was immobilized effectively on PMMA surface by covalent binding with maleic anhydride groups. The cover was treated with plasma treatment to improve the hydrophilic ability, so that liquid could flow smoothly in the microchannel. The whole analysis process could be completed within 10 min. There was a very good linear correlation between response and H-FABP concentration in the range of 0.5-100 ng/mL(R²=0.9966), and the detection limit was 0.1 ng/mL(S/N=3). The intra-assay precision(RSD) was less than 10% and the inter-assay RSD was less than 15%. This detection method has the advantages such as high sensitivity, fast detection and good accuracy.
- Heart-type fatty acid binding protein,
- Detection,
- Microfluidic system,
- Time-resolved fluorescence
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